Between the 1960s and 1980s, most life scientists focused their attention on studies of
nucleic acids and the translation of the coded information. Protein degradation was a …

E3 ligases confer specificity to ubiquitination by recognizing target substrates and mediating
transfer of ubiquitin from an E2 ubiquitin-conjugating enzyme to substrate. The activity of …

Cullin–RING complexes comprise the largest known class of ubiquitin ligases. Owing to the
great diversity of their substrate-receptor subunits, it is possible that there are hundreds of …

Progression through the eukaryotic cell cycle is known to be both regulated and
accompanied by periodic fluctuation in the expression levels of numerous genes. We report …

Ubiquitin is the founding member of a family of structurally conserved proteins that regulate
a host of processes in eukaryotic cells. Ubiquitin and its relatives carry out their functions …

The field of proteomics is built on technologies to analyze large numbers of proteins—ideally
the entire proteome—in the same experiment. Mass spectrometry (MS) has been …

Proteomics can be viewed as an experimental approach to explain the information
contained in genomic sequences in terms of the structure, function, and control of biological …

I discuss advances in the cell cycle in the 21 years since cyclin was discovered. The
surprising redundancy amongst the classical cyclins (A, B, and E) and cyclin-dependent …

The 26 S proteasome mediates degradation of ubiquitin-conjugated proteins. Although
ubiquitin is recycled from proteasome substrates, the molecular basis of deubiquitination at …

The interactions between proteins, DNA, and RNA in living cells constitute molecular
networks that govern various cellular functions. To investigate the global dynamical …