SELEX—A (r) evolutionary method to generate high-affinity nucleic acid ligands
SELEX stands for systematic evolution of ligands by exponential enrichment. This method,
described primarily in 1990 [Ellington, AD, Szostak, JW, 1990. In vitro selection of RNA …
described primarily in 1990 [Ellington, AD, Szostak, JW, 1990. In vitro selection of RNA …
Implications of peptide assemblies in amyloid diseases
Neurodegenerative disorders and type 2 diabetes are global epidemics compromising the
quality of life of millions worldwide, with profound social and economic implications. Despite …
quality of life of millions worldwide, with profound social and economic implications. Despite …
Immunoassays with rolling circle DNA amplification: a versatile platform for ultrasensitive antigen detection
B Schweitzer, S Wiltshire, J Lambert… - Proceedings of the …, 2000 - National Acad Sciences
We describe an adaptation of the rolling circle amplification (RCA) reporter system for the
detection of protein Ags, termed “immunoRCA.” In immunoRCA, an oligonucleotide primer is …
detection of protein Ags, termed “immunoRCA.” In immunoRCA, an oligonucleotide primer is …
[HTML][HTML] Aptamers used for biosensors and targeted therapy
Y Ning, J Hu, F Lu - Biomedicine & Pharmacotherapy, 2020 - Elsevier
Aptamers are single-stranded nucleic acid sequences that can bind to target molecules with
high selectivity and affinity. Most aptamers are screened in vitro by a combinatorial biology …
high selectivity and affinity. Most aptamers are screened in vitro by a combinatorial biology …
A G-quadruplex–containing RNA activates fluorescence in a GFP-like fluorophore
Spinach is an in vitro–selected RNA aptamer that binds a GFP-like ligand and activates its
green fluorescence. Spinach is thus an RNA analog of GFP and has potentially widespread …
green fluorescence. Spinach is thus an RNA analog of GFP and has potentially widespread …
Methods developed for SELEX
SCB Gopinath - Analytical and bioanalytical chemistry, 2007 - Springer
SELEX (systematic evolution of ligands by exponential enrichment) is a process that
involves the progressive purification from a combinatorial library of nucleic acid ligands with …
involves the progressive purification from a combinatorial library of nucleic acid ligands with …
DNA aptamers that bind to MUC1 tumour marker: design and characterization of MUC1-binding single-stranded DNA aptamers
CSM Ferreira, CS Matthews, S Missailidis - Tumor biology, 2006 - karger.com
Agents able to bind tightly and selectively to disease markers can greatly benefit disease
diagnosis and therapy. Aptamers are functional molecules, usually DNA or RNA …
diagnosis and therapy. Aptamers are functional molecules, usually DNA or RNA …
Prion protein recruits its neuronal receptor NCAM to lipid rafts to activate p59fyn and to enhance neurite outgrowth
A Santuccione, V Sytnyk, I Leshchyns' ka… - The Journal of cell …, 2005 - rupress.org
In spite of advances in understanding the role of the cellular prion protein (PrP) in neural cell
interactions, the mechanisms of PrP function remain poorly characterized. We show that PrP …
interactions, the mechanisms of PrP function remain poorly characterized. We show that PrP …
Nucleic acid aptamers from selection in vitro to applications in vivo
Aptamers are nucleic acid ligands which are isolated from combinatorial oligonucleotide
libraries by in vitro selection. They exhibit highly complex and sophisticated molecular …
libraries by in vitro selection. They exhibit highly complex and sophisticated molecular …
The emerging structural complexity of G-quadruplex RNAs
MT Banco, AR Ferré-D'Amaré - Rna, 2021 - rnajournal.cshlp.org
G-quadruplexes (G4s) are four-stranded nucleic acid structures that arise from the stacking
of G-quartets, cyclic arrangements of four guanines engaged in Hoogsteen base-pairing …
of G-quartets, cyclic arrangements of four guanines engaged in Hoogsteen base-pairing …