[HTML][HTML] Phosphoramidate Azole oligonucleotides for single nucleotide polymorphism detection by PCR

AS Chubarov, EE Baranovskaya, IP Oscorbin… - International Journal of …, 2024 - mdpi.com
Detection of the Kirsten rat sarcoma gene (KRAS) mutational status is an important factor for
the treatment of various malignancies. The most common KRAS-activating mutations are …

Allele-specific PCR for PIK3CA mutation detection using phosphoryl guanidine modified primers

AS Chubarov, IP Oscorbin, LM Novikova, ML Filipenko… - Diagnostics, 2023 - mdpi.com
Phosphoryl guanidine (PG) is the novel uncharged modification of internucleotide
phosphates of oligonucleotides. Incorporating PG modification into PCR primers leads to …

[HTML][HTML] Molecular effects of site-specific phosphate-methylated primer on the structure and motions of Taq DNA polymerase

YC Tsai, WY Chen, C Chiu - Computational and Structural Biotechnology …, 2023 - Elsevier
Polymerase chain reaction (PCR) is a powerful molecular biology assay for gene detection
and quantification. Conventional DNA primers for PCR often suffer from poor sensitivity in …

[PDF][PDF] Allele-specific PCR for PIK3CA mutation detection using phosphoryl guanidine modified primers. Diagnostics. 2023; 13: 250

AS Chubarov, IP Oscorbin, LM Novikova, ML Filipenko… - 2023 - researchgate.net
Phosphoryl guanidine (PG) is the novel uncharged modification of internucleotide
phosphates of oligonucleotides. Incorporating PG modification into PCR primers leads to …