One of the most salient features of Bacillus subtilis and related bacilli is their natural capacity
to secrete a variety of proteins into their environment, frequently to high concentrations. This …

The unifying feature of all proteins that are transported out of the cytoplasm of gram-negative
bacteria by the general secretory pathway (GSP) is the presence of a long stretch of …

A new strategy for predicting the topology of bacterial inner membrane proteins is proposed
on the basis of hydrophobicity analysis, automatic generation of a set of possible topologies …

S. aureus is resistant to various first-line antibiotics, and seeking multifarious strategies
aimed at effective control of antibiotic-resistant behavior is urgently needed. Here, we report …

The export of many E. coli proteins such as pmOmpA requires the cytosolic chaperone SecB
and the membrane-bound preprotein translocase. Translocase is a multisubunit enzyme …

We have previously reconstituted the soluble phase of precursor protein translocation in
vitro using purified proteins (the precursor proOmpA, the chaperone SecB, and the ATPase …

Covalent modification of membrane proteins with lipids appears to be ubiquitous in all living
cells. The major outer membrane (Braun's) lipoprotein of E. coli, the prototype of bacterial …

When secY is overexpressed over secE or secE is underexpressed, a fraction of SecY
protein is rapidly degraded in vivo. This proteolysis was unaffected in previously described …

Escherichia coli azi mutants, whose growth is resistant to millimolar concentrations of
sodium azide, were among the earliest E. coli mutants isolated. Genetic complementation …

We show here that the rate of protein translocation in the bacterium Escherichia coli
depends on the levels of the SecD and SecF proteins in the cell. Overexpression of SecD …