Directed evolution aims to expedite the natural evolution process of biological molecules
and systems in a test tube through iterative rounds of gene diversifications and library …

Directed evolution is one of the most powerful tools for protein engineering and functions by
harnessing natural evolution, but on a shorter timescale. It enables the rapid selection of …

Directed evolution has proved to be an effective strategy for improving or altering the activity
of biomolecules for industrial, research and therapeutic applications. The evolution of …

Directed evolution has advanced into a standard industrial “tool” to tailor naturally occurring
proteins for a variety of biotechnological applications, thus enabling product valorization and …

We have developed a protocol to assemble in one step and one tube at least nine separate
DNA fragments together into an acceptor vector, with 90% of recombinant clones obtained …

Yeasts have been used for thousands of years to make fermented foods and beverages,
such as beer, wine, sake, and bread. However, the choice for a particular yeast strain or …

From reviews to the first edtion:" Bornscheuer and Kazlauskas have set out, and succeeded,
in producing a definitive manual on hydrolytic enzymes (especially lipases, esterases, and …

Enzymes have the potential to catalyse a wide variety of chemical reactions. They are
increasingly being sought as environmentally friendly and cost‐effective alternatives to …

Health care authorities are calling for new antibacterial therapies to cope with the global
emergence of antibiotic-resistant bacteria. Bacteriophage-encoded lysins are a unique class …

Modern enzyme development relies to an increasing extent on strategies based on diversity
generation followed by screening for variants with optimised properties. In principle, these …