This review describes key milestones related to the production of biopharmaceuticals—
therapies manufactured using recombinant DNA technology. The market for …

Highlights•The CHO genome suffers from chromosomal rearrangements and other stability-
related issues.•The CHO cell instability phenotype is characterized by titer instability and …

The glutamine synthetase (GS)-based Chinese hamster ovary (CHO) selection system is an
attractive approach to efficiently identify suitable clones in the cell line generation process …

Mammalian cells have developed dedicated molecular mechanisms to tightly control
expression levels of their genes where the specific transcriptomic signature across all genes …

Computationally designed protein nanoparticles have recently emerged as a promising
platform for the development of new vaccines and biologics. For many applications …

Background Cell line-specific, genome-scale metabolic models enable rigorous and
systematic in silico investigation of cellular metabolism. Such models have recently become …

Drug resistance continues to impede the success of cancer treatments, creating a need for
experimental model systems that are broad, yet simple, to allow the identification of …

Host‐cell proteins (HCPs) are the foremost class of process‐related impurities to be
controlled and removed in downstream processing steps in monoclonal antibody (mAb) …

Mammalian expression platforms are primary production systems for therapeutic proteins
that require complex post-translational modifications. Current processes used for develo** …

Biologics represent the fastest growing group of therapeutics, but many advanced
recombinant protein moieties remain difficult to produce. Here, we identify metabolic …