Proteomic analysis of post-translational modifications

M Mann, ON Jensen - Nature biotechnology, 2003 - nature.com
Post-translational modifications modulate the activity of most eukaryote proteins. Analysis of
these modifications presents formidable challenges but their determination generates …

Modification-specific proteomics: characterization of post-translational modifications by mass spectrometry

ON Jensen - Current opinion in chemical biology, 2004 - Elsevier
Post-translational modifications generate tremendous diversity, complexity and
heterogeneity of gene products, and their determination is one of the main challenges in …

Peptide and protein sequence analysis by electron transfer dissociation mass spectrometry

JEP Syka, JJ Coon, MJ Schroeder… - Proceedings of the …, 2004 - National Acad Sciences
Peptide sequence analysis using a combination of gas-phase ion/ion chemistry and tandem
mass spectrometry (MS/MS) is demonstrated. Singly charged anthracene anions transfer an …

Analysis of protein phosphorylation using mass spectrometry: deciphering the phosphoproteome

M Mann, SE Ong, M Grønborg, H Steen… - Trends in …, 2002 - cell.com
In signal transduction in eukaryotes, protein phosphorylation is a key event. To understand
signaling processes, we must first acquire an inventory of phosphoproteins and their …

Interpreting the protein language using proteomics

ON Jensen - Nature reviews Molecular cell biology, 2006 - nature.com
Post-translational modifications define the functional and structural plasticity of proteins in
archaea, prokaryotes and eukaryotes. Multi-site protein modification modulates protein …

Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway* S

A Gruhler, JV Olsen, S Mohammed, P Mortensen… - Molecular & cellular …, 2005 - ASBMB
Cellular processes such as proliferation, differentiation, and adaptation to environmental
changes are regulated by protein phosphorylation. Development of sensitive and …

On the development of plasma protein biomarkers

S Surinova, R Schiess, R Hüttenhain… - Journal of proteome …, 2011 - ACS Publications
The development of plasma biomarkers has proven to be more challenging than initially
anticipated. Many studies have reported lists of candidate proteins rather than validated …

Current and prospective applications of metal ion–protein binding

EKM Ueda, PW Gout, L Morganti - Journal of chromatography A, 2003 - Elsevier
Since immobilized metal ion affinity chromatography (IMAC) was first introduced, several
variants of this method and many other metal affinity-based techniques have been devised …

Post-translational modifications and their biological functions: proteomic analysis and systematic approaches

JW Seo, KJ Lee - BMB Reports, 2004 - koreascience.kr
Recently produced information on post-translational modifications makes it possible to
interpret their biological regulation with new insights. Various protein modifications finely …

Phosphopeptide enrichment by aliphatic hydroxy acid-modified metal oxide chromatography for nano-LC-MS/MS in proteomics applications* S

N Sugiyama, T Masuda, K Shinoda, A Nakamura… - Molecular & Cellular …, 2007 - ASBMB
We developed novel methods for phosphopeptide enrichment using aliphatic hydroxy acid-
modified metal oxide chromatography (MOC). Titania and zirconia were successfully applied …