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Development of better aptamers: Structured library approaches, selection methods, and chemical modifications
Systematic evolution of ligands by exponential enrichment (SELEX) has been used to
discover thousands of aptamers since its development in 1990. Aptamers are short single …
discover thousands of aptamers since its development in 1990. Aptamers are short single …
Advances and challenges in small‐molecule DNA aptamer isolation, characterization, and sensor development
Aptamers are short oligonucleotides isolated in vitro from randomized libraries that can bind
to specific molecules with high affinity, and offer a number of advantages relative to …
to specific molecules with high affinity, and offer a number of advantages relative to …
Pushing adenosine and ATP SELEX for DNA aptamers with nanomolar affinity
The classical DNA aptamer for adenosine and ATP has been the most used small molecule
binding aptamer for biosensing, imaging, and DNA nanotechnology. This sequence has …
binding aptamer for biosensing, imaging, and DNA nanotechnology. This sequence has …
[HTML][HTML] Three decades of nucleic acid aptamer technologies: Lessons learned, progress and opportunities on aptamer development
Aptamers are short single-stranded nucleic acid sequences capable of binding to target
molecules in a way similar to antibodies. Due to various advantages such as prolonged …
molecules in a way similar to antibodies. Due to various advantages such as prolonged …
Guide to selecting a biorecognition element for biosensors
MA Morales, JM Halpern - Bioconjugate chemistry, 2018 - ACS Publications
Biosensors are powerful diagnostic tools defined as having a biorecognition element for
analyte specificity and a transducer for a quantifiable signal. There are a variety of different …
analyte specificity and a transducer for a quantifiable signal. There are a variety of different …
[HTML][HTML] Advances in aptameric biosensors designed to detect toxic contaminants from food, water, human fluids, and the environment
Detection of toxic small molecule contaminants with sensitivity, accuracy, and specificity is a
challenging task. Traditionally used HPLC and mass spectrometry-based assays suffer from …
challenging task. Traditionally used HPLC and mass spectrometry-based assays suffer from …
Investigations on the interface of nucleic acid aptamers and binding targets
Nucleic acid aptamers are single-stranded DNA or RNA of 20–100 nucleotides in length that
have attracted substantial scientific interest due to their ability to specifically bind to target …
have attracted substantial scientific interest due to their ability to specifically bind to target …
Dopamine and melamine binding to gold nanoparticles dominates their aptamer-based label-free colorimetric sensing
Target-directed aptamer adsorption by gold nanoparticles (AuNPs) has been widely used to
develop label-free colorimetric biosensors. However, the potential interactions between …
develop label-free colorimetric biosensors. However, the potential interactions between …
Perspective on the future role of aptamers in analytical chemistry
It has been almost 30 years since the invention of Systematic Evolution of Ligands by
Exponential Enrichment (SELEX) methodology and the description of the first aptamers. In …
Exponential Enrichment (SELEX) methodology and the description of the first aptamers. In …
Assays to estimate the binding affinity of aptamers
Aptamers have become coming-of-age molecular recognition elements in both diagnostic
and therapeutic applications. Generated by SELEX, the 'quality control'of aptamers, which …
and therapeutic applications. Generated by SELEX, the 'quality control'of aptamers, which …