CRISPR/Cas systems in genome editing: methodologies and tools for sgRNA design, off‐target evaluation, and strategies to mitigate off‐target effects
Life sciences have been revolutionized by genome editing (GE) tools, including zinc finger
nucleases, transcription activator‐Like effector nucleases, and CRISPR (clustered regulatory …
nucleases, transcription activator‐Like effector nucleases, and CRISPR (clustered regulatory …
[HTML][HTML] Construct design for CRISPR/Cas-based genome editing in plants
CRISPR construct design is a key step in the practice of genome editing, which includes
identification of appropriate Cas proteins, design and selection of guide RNAs (gRNAs), and …
identification of appropriate Cas proteins, design and selection of guide RNAs (gRNAs), and …
[HTML][HTML] A review of emerging physical transfection methods for CRISPR/Cas9-mediated gene editing
Gene editing is a versatile technique in biomedicine that promotes fundamental research as
well as clinical therapy. The development of Clustered Regularly Interspaced Short …
well as clinical therapy. The development of Clustered Regularly Interspaced Short …
A compact Cas9 ortholog from Staphylococcus Auricularis (SauriCas9) expands the DNA targeting scope
Z Hu, S Wang, C Zhang, N Gao, M Li, D Wang… - PLoS …, 2020 - journals.plos.org
Compact CRISPR/Cas9 systems that can be packaged into an adeno-associated virus
(AAV) hold great promise for gene therapy. Unfortunately, currently available small Cas9 …
(AAV) hold great promise for gene therapy. Unfortunately, currently available small Cas9 …
Optimized design parameters for CRISPR Cas9 and Cas12a homology-directed repair
CRISPR–Cas proteins are RNA-guided nucleases used to introduce double-stranded
breaks (DSBs) at targeted genomic loci. DSBs are repaired by endogenous cellular …
breaks (DSBs) at targeted genomic loci. DSBs are repaired by endogenous cellular …
Homozygous might be hemizygous: CRISPR/Cas9 editing in iPSCs results in detrimental on-target defects that escape standard quality controls
The ability to precisely edit the genome of human induced pluripotent stem cell (iPSC) lines
using CRISPR/Cas9 has enabled the development of cellular models that can address …
using CRISPR/Cas9 has enabled the development of cellular models that can address …
Harnessing the evolving CRISPR/Cas9 for precision oncology
T Li, S Li, Y Kang, J Zhou, M Yi - Journal of Translational Medicine, 2024 - Springer
Abstract The Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/Cas9
system, a groundbreaking innovation in genetic engineering, has revolutionized our …
system, a groundbreaking innovation in genetic engineering, has revolutionized our …
NmeCas9 is an intrinsically high-fidelity genome-editing platform
Background The development of CRISPR genome editing has transformed biomedical
research. Most applications reported thus far rely upon the Cas9 protein from Streptococcus …
research. Most applications reported thus far rely upon the Cas9 protein from Streptococcus …
Decorating chromatin for enhanced genome editing using CRISPR-Cas9
CRISPR-associated (Cas) enzymes have revolutionized biology by enabling RNA-guided
genome editing. Homology-directed repair (HDR) in the presence of donor templates is …
genome editing. Homology-directed repair (HDR) in the presence of donor templates is …
In planta gene targeting can be enhanced by the use of CRISPR/Cas12a
The controlled change of plant genomes by homologous recombination (HR) is still difficult
to achieve. We previously developed the in planta gene targeting (ipGT) technology which …
to achieve. We previously developed the in planta gene targeting (ipGT) technology which …