The amino acid sequence of a protein affects both its structure and its function. Thus, the
ability to modify the sequence, and hence the structure and activity, of individual proteins in …

Immunoassays are reliable, efficient, and accurate methods for the analysis of small‐
molecule harmful substances (such as pesticides, veterinary drugs, and biological toxins) …

The ability to engineer biological systems, whether to introduce novel functionality or
improved performance, is a cornerstone of biotechnology and synthetic biology. Typically …

Gene-directed enzyme-prodrug therapy (GDEPT) is a promising anti-cancer strategy.
However, inadequate prodrugs, inefficient prodrug activation, and a lack of non-invasive …

Here, we describe the successful engineering of a promiscuous glucose dehydrogenase
from the thermophilic archaeon, Saccharolobus solfataricus, toward the thermostable and …

Saturation mutagenesis is a powerful technique for engineering proteins, metabolic
pathways and genomes. In spite of its numerous applications, creating high-quality …

Genetic engineering for heterologous expression has advanced in recent years. Model
systems such as Escherichia coli, Bacillus subtilis and Pichia pastoris are often used as host …

Among all congenital cataracts caused by genetic mutations, approximately half are caused
by a mutation in crystallin genes, and accounts the leading cause of blindness in children …

Epitope map** is a technique employed to define the region of an antigen that elicits an
immune response, providing crucial insight into the structural architecture of the antigen as …

With the goal of generating new enzymes that can cleave custom sequences, this article
describes a selection strategy for evolving proteases with desirable characteristics. Positive …