RNA polymerase II speed: a key player in controlling and adapting transcriptome composition

L Muniz, E Nicolas, D Trouche - The EMBO journal, 2021‏ - embopress.org
Abstract RNA polymerase II (RNA Pol II) speed or elongation rate, ie, the number of
nucleotides synthesized per unit of time, is a major determinant of transcriptome …

[HTML][HTML] Causes and consequences of hematopoietic stem cell heterogeneity

S Haas, A Trumpp, MD Milsom - Cell stem cell, 2018‏ - cell.com
Blood and immune cells derive from multipotent hematopoietic stem cells (HSCs).
Classically, stem and progenitor populations have been considered discrete homogeneous …

Dynamic imaging of RNA in living cells by CRISPR-Cas13 systems

LZ Yang, Y Wang, SQ Li, RW Yao, PF Luan, H Wu… - Molecular cell, 2019‏ - cell.com
Visualizing the location and dynamics of RNAs in live cells is key to understanding their
function. Here, we identify two endonuclease-deficient, single-component programmable …

Dynamic interplay between enhancer–promoter topology and gene activity

H Chen, M Levo, L Barinov, M Fujioka, JB Jaynes… - Nature …, 2018‏ - nature.com
A long-standing question in gene regulation is how remote enhancers communicate with
their target promoters, and specifically how chromatin topology dynamically relates to gene …

Illuminating RNA biology through imaging

P Le, N Ahmed, GW Yeo - Nature Cell Biology, 2022‏ - nature.com
RNA processing plays a central role in accurately transmitting genetic information into
functional RNA and protein regulators. To fully appreciate the RNA life-cycle, tools to …

Enhancer control of transcriptional bursting

T Fukaya, B Lim, M Levine - Cell, 2016‏ - cell.com
Transcription is episodic, consisting of a series of discontinuous bursts. Using live-imaging
methods and quantitative analysis, we examine transcriptional bursting in living Drosophila …

Transcription in living cells: molecular mechanisms of bursting

J Rodriguez, DR Larson - Annual review of biochemistry, 2020‏ - annualreviews.org
Transcription in several organisms from certain bacteria to humans has been observed to be
stochastic in nature: toggling between active and inactive states. Periods of active nascent …

Translation dynamics of single mRNAs in live cells and neurons

B Wu, C Eliscovich, YJ Yoon, RH Singer - Science, 2016‏ - science.org
Translation is the fundamental biological process converting mRNA information into
proteins. Single-molecule imaging in live cells has illuminated the dynamics of RNA …

Synthetic recording and in situ readout of lineage information in single cells

KL Frieda, JM Linton, S Hormoz, J Choi, KHK Chow… - Nature, 2017‏ - nature.com
Reconstructing the lineage relationships and dynamic event histories of individual cells
within their native spatial context is a long-standing challenge in biology. Many biological …

Nascent RNA analyses: tracking transcription and its regulation

EM Wissink, A Vihervaara, ND Tippens… - Nature Reviews …, 2019‏ - nature.com
The programmes that direct an organism's development and maintenance are encoded in its
genome. Decoding of this information begins with regulated transcription of genomic DNA …