Hepatocytes, like other epithelia, are situated at the interface between the organism's
exterior and the underlying internal milieu and organize the vectorial exchange of …

Positioned at the C-terminus of many eukaryotic proteins, the glycosylphosphatidylinositol
(GPI) anchor is a posttranslational modification that anchors the modified protein in the outer …

Abstract Background In 2017, the US Food and Drug Administration initiated expansion of
drug labels for the treatment of cystic fibrosis (CF) to include CF transmembrane …

GPI-linked protein molecules become Triton-insoluble during polarized sorting to the apical
cell surface of epithelial cells. These insoluble complexes, enriched in cholesterol …

Yeaman, Charles, Kent K. Grindstaff, and W. James Nelson. New Perspectives on
Mechanisms Involved in Generating Epithelial Cell Polarity. Physiol. Rev. 79: 73–98, 1999 …

Caveolae are subdomains of the plasma membrane which concentrate cholesterol,
glycosphingolipids, and glycosylphosphatidylinositol-linked proteins. It has recently been …

In the early days of epithelial cell biology, researchers working with kidney and/or intestinal
epithelial cell lines and with hepatocytes described the biosynthetic and recycling routes …

The ultrastructural distribution of the ganglioside GM1 was investigated in A431 cells. After
fixation, the cells were frozen in liquid nitrogen, freeze-substituted, and then embedded in …

The endocytic itineraries of lipid raft markers, such as glycosyl phosphatidylinositol (GPI)-
anchored proteins and glycosphingolipids, are incompletely understood. Here we show that …

Glycosylphosphatidylinositol (GPI) anchoring of proteins is a conserved post-translational
modification in eukaryotes. GPI is synthesized and transferred to proteins in the endoplasmic …