Cryogenic electron microscopy and data processing enable the determination of structures
of isolated macromolecules to near-atomic resolution. However, these data do not provide …

Recent advances in cryo-electron microscopy have marked only the beginning of the
potential of this technique. To bring structure into cell biology, the modality of cryo-electron …

Single-particle cryo-electron microscopy has become a widely adopted method in structural
biology due to many recent technological advances in microscopes, detectors and image …

We present a bimodal endocytic tracer, fluorescent BSA-gold (fBSA-Au), as a fiducial marker
for 2D and 3D correlative light and electron microscopy (CLEM) applications. fBSA-Au …

Over the last few years, cryo electron microscopy has become the most important method in
structural biology. While 80% of deposited maps are from single particle analysis, electron …

In situ cryo-Electron Microscopy (cryo-EM) enables the direct interrogation of structure-
function relationships by resolving macromolecular structures in their native cellular …

Biological carbohydrate polymers represent some of the most complex molecules in life,
enabling their participation in a huge range of physiological functions. The complexity of …

Electron tomography entails the three-dimensional reconstruction of an object from its
projections, which are acquired using a transmission electron microscope (TEM). This …