In the past few years, significant technological breakthroughs in single particle cryo-electron
microscopy enabled a 'resolution revolution'of this technique. It also changed structural …

Highlights•Cryo-EM is a powerful tool to investigate the structures of membrane
proteins.•Recent technological advancements in the field of cryo-EM have enabled structure …

Olfactory systems must detect and discriminate amongst an enormous variety of odorants.
To contend with this challenge, diverse species have converged on a common strategy in …

Integral membrane protein structure determination traditionally requires extraction from cell
membranes using detergents or polymers. Here, we describe the isolation and structure …

Transient receptor potential vanilloid member 1 (TRPV1) is a Ca2+-permeable cation
channel that serves as the primary heat and capsaicin sensor in humans. Using cryo-EM, we …

Pain therapy has remained conceptually stagnant since the opioid crisis, which highlighted
the dangers of treating pain with opioids. An alternative addiction-free strategy to …

Small molecule chaperones have been exploited as therapeutics for the hundreds of
diseases caused by protein misfolding. The most successful examples are the CFTR …

INTRODUCTION The nine subtypes of mammalian voltage-gated sodium (Nav) channels,
Nav1. 1 to Nav1. 9, are responsible for the initiation and propagation of action potentials in …

Voltage-gated sodium channel Nav1. 7 represents a promising target for pain relief. Here we
report the cryo–electron microscopy structures of the human Nav1. 7-β1-β2 complex bound …

OSCA/TMEM63s form mechanically activated (MA) ion channels in plants and animals,
respectively. OSCAs and related TMEM16s and transmembrane channel-like (TMC) …