[HTML][HTML] Electron paramagnetic resonance spectroscopy in structural-dynamic studies of large protein complexes

L Galazzo, E Bordignon - Progress in nuclear magnetic resonance …, 2023 - Elsevier
Macromolecular protein assemblies are of fundamental importance for many processes
inside the cell, as they perform complex functions and constitute central hubs where …

Pulsed dipolar EPR spectroscopy and metal ions: methodology and biological applications

D Abdullin, O Schiemann - ChemPlusChem, 2020 - Wiley Online Library
Pulsed dipolar electron paramagnetic resonance spectroscopy (PDS) is a valuable method
for determining biomolecular structures and their conformational changes during function …

Sub‐Micromolar Pulse Dipolar EPR Spectroscopy Reveals Increasing CuII‐labelling of Double‐Histidine Motifs with Lower Temperature

JL Wort, K Ackermann, A Giannoulis… - Angewandte …, 2019 - Wiley Online Library
Electron paramagnetic resonance (EPR) distance measurements are making increasingly
important contributions to the studies of biomolecules by providing highly accurate …

Nanomolar Pulse Dipolar EPR Spectroscopy in Proteins: CuII–CuII and Nitroxide–Nitroxide Cases

K Ackermann, JL Wort, BE Bode - The Journal of Physical …, 2021 - ACS Publications
The study of ever more complex biomolecular assemblies implicated in human health and
disease is facilitated by a suite of complementary biophysical methods. Pulse dipolar …

RIDME Spectroscopy: New Topics Beyond the Determination of Electron Spin–Spin Distances

S Kuzin, M Yulikov - The Journal of Physical Chemistry Letters, 2025 - ACS Publications
Relaxation-induced dipolar modulation enhancement (RIDME) is a pulse EPR experiment
originally designed to determine distances between spin labels. However, RIDME has …

[HTML][HTML] Neural networks in pulsed dipolar spectroscopy: A practical guide

J Keeley, T Choudhury, L Galazzo, E Bordignon… - Journal of Magnetic …, 2022 - Elsevier
This is a methodological guide to the use of deep neural networks in the processing of
pulsed dipolar spectroscopy (PDS) data encountered in structural biology, organic …

Spectroscopically orthogonal labelling to disentangle site-specific nitroxide label distributions

V Vitali, K Ackermann, G Hagelueken… - Applied Magnetic …, 2024 - Springer
Biomolecular applications of pulse dipolar electron paramagnetic resonance spectroscopy
(PDS) are becoming increasingly valuable in structural biology. Site-directed spin labelling …

[HTML][HTML] UWB DEER and RIDME distance measurements in Cu (II)–Cu (II) spin pairs

FD Breitgoff, K Keller, M Qi, D Klose, M Yulikov… - Journal of Magnetic …, 2019 - Elsevier
Abstract Distance determination by Electron Paramagnetic Resonance (EPR) based on
measurements of the dipolar coupling are technically challenging for electron spin systems …

A general model to optimise Cu II labelling efficiency of double-histidine motifs for pulse dipolar EPR applications

JL Wort, K Ackermann, DG Norman… - Physical Chemistry …, 2021 - pubs.rsc.org
Electron paramagnetic resonance (EPR) distance measurements are making increasingly
important contributions to studies of biomolecules underpinning health and disease by …

Direct Comparison between Förster Resonance Energy Transfer and Light-Induced Triplet–Triplet Electron Resonance Spectroscopy

A Bertran, L Morbiato, J Sawyer… - Journal of the …, 2023 - ACS Publications
To carry out reliable and comprehensive structural investigations, the exploitation of different
complementary techniques is required. Here, we report that dual triplet-spin/fluorescent …