The 3D organization of mammalian chromatin was described more than 30 years ago by
visualizing sites of DNA synthesis at different times during the S phase of the cell cycle …

It has been 8 years since the concept of naïve and primed pluripotent stem cell states was
first proposed. Both are states of pluripotency, but exhibit slightly different properties. The …

Eukaryotic chromosomes replicate in a temporal order known as the replication-timing
program. In mammals, replication timing is cell-type-specific with at least half the genome …

DNA replication enables genetic inheritance across the kingdoms of life. Replication occurs
with a defined temporal order known as the replication timing (RT) programme, leading to …

Our current understanding of how DNA is packed in the nucleus is most accurate at the fine
scale of individual nucleosomes and at the large scale of chromosome territories. However …

The laboratory mouse shares the majority of its protein-coding genes with humans, making it
the premier model organism in biomedical research, yet the two mammals differ in …

The temporal order of DNA replication (replication timing [RT]) is highly coupled with
genome architecture, but cis-elements regulating either remain elusive. We created a series …

This protocol is an extension to: Nat. Protoc. 6, 870–895 (2014); doi: 10.1038/nprot.
2011.328; published online 02 June 2011 Cycling cells duplicate their DNA content during S …

Duplication of the genome in mammalian cells occurs in a defined temporal order referred to
as its replication-timing (RT) program. RT changes dynamically during development …

For more than three decades, investigators have sought to identify the precise locations
where DNA replication initiates in mammalian genomes. The development of molecular and …