Tutorial: practical considerations for tissue clearing and imaging

KR Weiss, FF Voigt, DP Shepherd, J Huisken - Nature protocols, 2021 - nature.com
Tissue clearing has become a powerful technique for studying anatomy and morphology at
scales ranging from entire organisms to subcellular features. With the recent proliferation of …

Inhibition of arginase modulates T-cell response in the tumor microenvironment of lung carcinoma

A Sosnowska, J Chlebowska-Tuz, P Matryba… - …, 2021 - Taylor & Francis
Immunotherapy has demonstrated significant activity in a broad range of cancer types, but
still the majority of patients receiving it do not maintain durable therapeutic responses …

Perspective: extending the utility of three-dimensional organoids by tissue clearing technologies

EA Susaki, M Takasato - Frontiers in cell and developmental biology, 2021 - frontiersin.org
An organoid, a self-organizing organ-like tissue developed from stem cells, can exhibit a
miniaturized three-dimensional (3D) structure and part of the physiological functions of the …

A 3D analysis of cleared human melanoma

V Llorente, D Sanderson, A Martín-Gorgojo… - Biomedicines, 2022 - mdpi.com
Cutaneous melanoma is one of the most aggressive and deadliest cancers in human beings
due to its invasiveness and other factors. Histopathological analysis is crucial for a proper …

Tissue optical clearing and expanding solution for improved nuclear segmentation

P Matryba, A Wolny, A Chwastowicz, J Tomczuk… - View, 2025 - Wiley Online Library
Over the recent years, fluorescence microscopy has seen substantial progress, transitioning
from primarily qualitative analysis to a profoundly quantitative methodology, yet limitations in …

A simple and effective vascular network labeling method for transparent tissues of mice

L Bai, Y Wu, W Dai, J Zhang, L Zheng… - Journal of …, 2023 - Wiley Online Library
Vascular network labeling in transparent tissues provides more complete information on
blood vessels. To achieve a fast and efficient method for vascular network labeling in …

[HTML][HTML] Imaging of fluorescent polymer dots in relation to channels and immune cells in the lymphatic system

Y Zhang, J Zhang, X Li, J Li, S Lu, Y Li, P Ren… - Materials Today Bio, 2022 - Elsevier
Polymer dots (Pdots) have been applied to imaging lymph nodes (LNs) and lymphatic
vessels (LVs) in living mice and rats. However, the mechanism of absorption, distribution …

Challenges and opportunities in hydrophilic tissue clearing methods

EA Susaki, HR Ueda - Handbook of Tissue Optical Clearing, 2022 - taylorfrancis.com
Hydrophilic tissue clearing protocols represent one of the major categories of recently
emerging optical tissue clearing methods. For over 20 years, researchers have tested many …

Semi-automated protocol to quantify and characterize fluorescent three-dimensional vascular images

DF **e, C Crouzet, K LoPresti, Y Wang, C Robinson… - Plos one, 2024 - journals.plos.org
The microvasculature facilitates gas exchange, provides nutrients to cells, and regulates
blood flow in response to stimuli. Vascular abnormalities are an indicator of pathology for …

Semi-automated protocol to quantify and characterize fluorescent, 3D vascular images

DF **e, C Crouzet, K LoPresti, Y Wang, C Robinson… - bioRxiv, 2022 - biorxiv.org
The microvasculature facilitates gas exchange, provides nutrients to cells, and regulates
blood flow in response to stimuli. Vascular abnormalities are an indicator of pathology for …