Metagenomic analyses: past and future trends

C Simon, R Daniel - Applied and environmental microbiology, 2011 - Am Soc Microbiol
Metagenomics has revolutionized microbiology by paving the way for a cultivation-
independent assessment and exploitation of microbial communities present in complex …

Metagenomics: tools and insights for analyzing next-generation sequencing data derived from biodiversity studies

A Oulas, C Pavloudi, P Polymenakou… - … and biology insights, 2015 - journals.sagepub.com
Advances in next-generation sequencing (NGS) have allowed significant breakthroughs in
microbial ecology studies. This has led to the rapid expansion of research in the field and …

CPC2: a fast and accurate coding potential calculator based on sequence intrinsic features

YJ Kang, DC Yang, L Kong, M Hou… - Nucleic acids …, 2017 - academic.oup.com
With advances in next-generation sequencing technologies, numerous novel transcripts in a
large number of organisms have been identified. With the goal of fast, accurate assessment …

Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy

Q Wang, GM Garrity, JM Tiedje… - Applied and …, 2007 - Am Soc Microbiol
ABSTRACT The Ribosomal Database Project (RDP) Classifier, a naïve Bayesian
classifier, can rapidly and accurately classify bacterial 16S rRNA sequences into the new …

Environmental genome shotgun sequencing of the Sargasso Sea

JC Venter, K Remington, JF Heidelberg, AL Halpern… - science, 2004 - science.org
We have applied “whole-genome shotgun sequencing” to microbial populations collected
en masse on tangential flow and impact filters from seawater samples collected from the …

Development of the human infant intestinal microbiota

C Palmer, EM Bik, DB DiGiulio, DA Relman… - PLoS …, 2007 - journals.plos.org
Almost immediately after a human being is born, so too is a new microbial ecosystem, one
that resides in that person's gastrointestinal tract. Although it is a universal and integral part …

Reduced diversity of faecal microbiota in Crohn's disease revealed by a metagenomic approach

C Manichanh, L Rigottier-Gois, E Bonnaud, K Gloux… - Gut, 2006 - gut.bmj.com
Background and aim: A role for the intestinal microbial community (microbiota) in the onset
and chronicity of Crohn's disease (CD) is strongly suspected. However, investigation of such …

Genomic insights that advance the species definition for prokaryotes

KT Konstantinidis, JM Tiedje - Proceedings of the National …, 2005 - National Acad Sciences
To help advance the species definition for prokaryotes, we have compared the gene content
of 70 closely related and fully sequenced bacterial genomes to identify whether species …

Critical evaluation of two primers commonly used for amplification of bacterial 16S rRNA genes

JA Frank, CI Reich, S Sharma… - Applied and …, 2008 - Am Soc Microbiol
ABSTRACT rRNA-based studies, which have become the most common method for
assessing microbial communities, rely upon faithful amplification of the corresponding genes …

Specific microbiome-dependent mechanisms underlie the energy harvest efficiency of ruminants

SKB Shabat, G Sasson, A Doron-Faigenboim… - The ISME …, 2016 - academic.oup.com
Ruminants have the remarkable ability to convert human-indigestible plant biomass into
human-digestible food products, due to a complex microbiome residing in the rumen …