Protein-protein interaction (PPI) is a hot topic in clinical research as protein networking has a
major impact in human disease. Such PPIs are potential drugs targets, leading to the need …

Recombinant proteins expressed as bacterial inclusion bodies (IBs) are now receiving
tremendous attention for many diverse applications in the areas of industrial and medical …

When the first version of this unit was written in 1995, protein purification of recombinant
proteins was based on a variety of standard chromatographic methods and approaches …

The production of high yields of soluble recombinant protein is one of the main objectives of
protein biotechnology. Several factors, such as expression system, vector, host, media …

Exosomes, a subpopulation of Extracellular vesicles (EVs), are cell-secreted vesicles found
in the majority of biological fluids, including breast milk, tears, sweat, blood and, urine. The …

Recombinant flounder growth hormone was overproduced in E. coli by using codon
optimized synthetic gene and optimized expression conditions for high level production. The …

Refolding of proteins derived from inclusion bodies is very promising as it can provide a
reliable source of target proteins of high purity. However, inclusion body-based protein …

Heterologous functional expression of the recombinant lipases is typically a bottleneck due
to the expression in the insoluble fraction as inclusion bodies (IBs) which are in inactive …

Background Mild solubilization of inclusion bodies has attracted attention in recent days,
with an objective to preserve the existing native-like secondary structure of proteins, reduce …

Diagnosing Zika virus (ZIKV) infections has been challenging due to the cross-reactivity of
induced antibodies with other flavivirus. The concomitant occurrence of ZIKV and Dengue …