Dynamic nuclear polarization for sensitivity enhancement in biomolecular solid-state NMR

T Biedenbänder, V Aladin, S Saeidpour… - Chemical …, 2022 - ACS Publications
Solid-state NMR with magic-angle spinning (MAS) is an important method in structural
biology. While NMR can provide invaluable information about local geometry on an atomic …

[HTML][HTML] Dynamic nuclear polarization for sensitivity enhancement in modern solid-state NMR

ASL Thankamony, JJ Wittmann, M Kaushik… - Progress in nuclear …, 2017 - Elsevier
The field of dynamic nuclear polarization has undergone tremendous developments and
diversification since its inception more than 6 decades ago. In this review we provide an in …

Biomolecular and biological applications of solid-state NMR with dynamic nuclear polarization enhancement

WY Chow, G De Paëpe, S Hediger - Chemical Reviews, 2022 - ACS Publications
Solid-state NMR spectroscopy (ssNMR) with magic-angle spinning (MAS) enables the
investigation of biological systems within their native context, such as lipid membranes, viral …

High-resolution NMR studies of antibiotics in cellular membranes

J Medeiros-Silva, S Jekhmane, AL Paioni… - Nature …, 2018 - nature.com
The alarming rise of antimicrobial resistance requires antibiotics with unexploited
mechanisms. Ideal templates could be antibiotics that target the peptidoglycan precursor …

DNP NMR of biomolecular assemblies

K Jaudzems, T Polenova, G Pintacuda… - Journal of structural …, 2019 - Elsevier
Abstract Dynamic Nuclear Polarization (DNP) is an effective approach to alleviate the
inherently low sensitivity of solid-state NMR (ssNMR) under magic angle spinning (MAS) …

Gd (III) and Mn (II) complexes for dynamic nuclear polarization: small molecular chelate polarizing agents and applications with site-directed spin labeling of proteins

M Kaushik, T Bahrenberg, TV Can… - Physical Chemistry …, 2016 - pubs.rsc.org
We investigate complexes of two paramagnetic metal ions Gd3+ and Mn2+ to serve as
polarizing agents for solid-state dynamic nuclear polarization (DNP) of 1H, 13C, and 15N at …

Targeted DNP for biomolecular solid-state NMR

D Gauto, O Dakhlaoui, I Marin-Montesinos… - Chemical …, 2021 - pubs.rsc.org
High-field dynamic nuclear polarization is revolutionizing the scope of solid-state NMR with
new applications in surface chemistry, materials science and structural biology. In this …

Applications of solid-state NMR to membrane proteins

V Ladizhansky - Biochimica et biophysica acta (BBA)-Proteins and …, 2017 - Elsevier
Membrane proteins mediate flow of molecules, signals, and energy between cells and
intracellular compartments. Understanding membrane protein function requires a detailed …

Structural biology applications of solid state MAS DNP NMR

Ü Akbey, H Oschkinat - Journal of Magnetic Resonance, 2016 - Elsevier
Abstract Dynamic Nuclear Polarization (DNP) has long been an aim for increasing sensitivity
of nuclear magnetic resonance (NMR) spectroscopy, delivering spectra in shorter …

Selective protein hyperpolarization in cell lysates using targeted dynamic nuclear polarization

T Viennet, A Viegas, A Kuepper, S Arens… - Angewandte …, 2016 - Wiley Online Library
Nuclear magnetic resonance (NMR) spectroscopy has the intrinsic capabilities to investigate
proteins in native environments. In general, however, NMR relies on non‐natural protein …