Alternative splicing of mRNA precursors is a nearly ubiquitous and extremely flexible point of
gene control in humans. It provides cells with the opportunity to create protein isoforms of …

Significant advances have been made in elucidating the biogenesis pathway and three-
dimensional structure of the UsnRNPs, the building blocks of the spliceosome. U2 and …

We have developed a generic procedure to purify proteins expressed at their natural level
under native conditions using a novel tandem affinity purification (TAP) tag. The TAP tag …

In a previous proteomic study of the human spliceosome, we identified 42 spliceosome-
associated factors, including 19 novel ones. Using enhanced mass spectrometric tools and …

Efficient targeted control of splicing is a major goal of functional genomics and therapeutic
applications. Guide (g) RNA-directed, deactivated (d) Cas CRISPR enzymes fused to …

A remarkably large collection of evolutionarily conserved proteins has been implicated in
processing of noncoding RNAs and biogenesis of ribonucleoproteins. To better define the …

Large-scale DNA sequencing has stimulated the development of proteomics by providing a
sequence infrastructure for protein analysis. Rapid and automated protein identification can …

▪ Abstract Introns are removed from precursor messenger RNAs in the cell nucleus by a
large ribonucleoprotein complex called the spliceosome. The spliceosome contains five …

Transcription is a source of genetic instability that can notably result from the formation of
genotoxic DNA: RNA hybrids, or R-loops, between the nascent mRNA and its template. Here …

Genomic sequences and expressed sequence tag data for a diverse group of fungi
(Saccharomyces cerevisiae, Schizosaccharomyces pombe, Aspergillus nidulans …