Agrobacterium-mediated transformation as a tool for functional genomics in fungi

CB Michielse, PJJ Hooykaas, CA van den Hondel… - Current genetics, 2005 - Springer
In the era of functional genomics, the need for tools to perform large-scale targeted and
random mutagenesis is increasing. A potential tool is Agrobacterium-mediated fungal …

Approaches to functional genomics in filamentous fungi

RJ Weld, KM Plummer, MA Carpenter, HJ Ridgway - Cell research, 2006 - nature.com
The study of gene function in filamentous fungi is a field of research that has made great
advances in very recent years. A number of transformation and gene manipulation strategies …

10 entomopathogenic fungi and their role in pest control

AK Charnley, SA Collins - Environmental and microbial relationships, 2007 - Springer
Synthetic chemical pesticides have been the mainstay of insect pest control for over 50
years. However, insecticide resistance, pest resurgence and concern over the …

A case of promiscuity: Agrobacterium's endless hunt for new partners

B Lacroix, T Tzfira, A Vainstein, V Citovsky - TRENDS in Genetics, 2006 - cell.com
Agrobacterium tumefaciens is a phytopathogenic bacterium that induces the 'crown
gall'disease in plants by transfer and integration of a segment of its tumor-inducing (Ti) …

CRISPR/Cas9-mediated efficient genome editing via blastospore-based transformation in entomopathogenic fungus Beauveria bassiana

J Chen, Y Lai, L Wang, S Zhai, G Zou, Z Zhou, C Cui… - Scientific reports, 2017 - nature.com
Beauveria bassiana is an environmentally friendly alternative to chemical insecticides
against various agricultural insect pests and vectors of human diseases. However, its …

Members of chitin synthase family in Metarhizium acridum differentially affect fungal growth, stress tolerances, cell wall integrity and virulence

J Zhang, H Jiang, Y Du, NO Keyhani, Y **a… - PLoS …, 2019 - journals.plos.org
Chitin is an important component of the fungal cell wall with a family of chitin synthases
mediating its synthesis. Here, we report on the genetic characterization of the full suite of …

Late Stage Oxidations during the Biosynthesis of the 2-Pyridone Tenellin in the Entomopathogenic Fungus Beauveria bassiana

LM Halo, MN Heneghan, AA Yakasai… - Journal of the …, 2008 - ACS Publications
Late stage oxidations during the biosynthesis of the 2-pyridone tenellin in the insect
pathogenic fungus Beauveria bassiana were investigated by a combination of gene …

Development of a simple and efficient transformation system for the basidiomycetous medicinal fungus Ganoderma lucidum

L Shi, X Fang, M Li, D Mu, A Ren, Q Tan… - World journal of …, 2012 - Springer
In this study, we report the development of a simple and efficient system for genetic
transformation of the medicinal fungus Ganoderma lucidum. Various parameters were …

Novel blastospore-based transformation system for integration of phosphinothricin resistance and green fluorescence protein genes into Beauveria bassiana

SH Ying, MG Feng - Applied microbiology and biotechnology, 2006 - Springer
A novel system was developed for efficient transformation of the fungal biocontrol agent
Beauveria bassiana. Competent blastospores were prepared and stored in LiAc-and …

Fungal type I polyketide synthases

RJ Cox, TJ Simpson - Methods in enzymology, 2009 - Elsevier
Fungi produce a wide variety of biologically active compounds, a large proportion of which
are produced by the polyketide biosynthetic pathway. Fungal polyketides comprise a very …