Directed evolution aims to expedite the natural evolution process of biological molecules
and systems in a test tube through iterative rounds of gene diversifications and library …

The incorporation of a synthetic, catalytically competent metallocofactor into a protein
scaffold to generate an artificial metalloenzyme (ArM) has been explored since the late …

The advent of modern C–H functionalization chemistries has enabled medicinal chemists to
consider a synthetic strategy, late stage functionalization (LSF), which utilizes the C–H …

Enzymes are versatile catalysts and their synthetic potential has been recognized for a long
time. In order to exploit their full potential, enzymes often need to be re-engineered or …

The field of biocatalysis has advanced from harnessing natural enzymes to using directed
evolution to obtain new biocatalysts with tailor-made functions. Several tools have recently …

Metal complex catalysis, in which coordination compounds are designed as catalysts for
chemical transformations employing noncovalent interactions, is emerging as a powerful …

The combination of sequential biocatalytic reactions, via non-natural synthetic cascades, is a
rapidly develo** field and leads to the generation of complex valuable chemicals from …

Enzymes that contain metal ions—that is, metalloenzymes—possess the reactivity of a
transition metal centre and the potential of molecular evolution to modulate the reactivity and …

Natural enzymes contain highly evolved active sites that lead to fast rates and high
selectivities. Although artificial metalloenzymes have been developed that catalyze …

The two main strategies for enzyme engineering, directed evolution and rational design,
have found widespread applications in improving the intrinsic activities of proteins. Although …