Human platelet lysate: replacing fetal bovine serum as a gold standard for human cell propagation?

T Burnouf, D Strunk, MBC Koh, K Schallmoser - Biomaterials, 2016 - Elsevier
The essential physiological role of platelets in wound healing and tissue repair builds the
rationale for the use of human platelet derivatives in regenerative medicine. Abundant …

The mesenchymal stromal cells dilemma—does a negative phase III trial of random donor mesenchymal stromal cells in steroid-resistant graft-versus-host disease …

J Galipeau - Cytotherapy, 2013 - Elsevier
The use of cryopreserved unmatched allogeneic mesenchymal stromal cells (MSCs) for
treatment of steroid-resistant graft-versus-host disease has become medical practice in …

Fetal bovine serum (FBS): past–present–future

J Van der Valk, K Bieback, C Buta, B Cochrane… - ALTEX-Alternatives to …, 2018 - altex.org
The supplementation of culture medium with fetal bovine serum (FBS, also referred to as
“fetal calf serum”) is still common practice in cell culture applications. Due to a number of …

A comparative analysis of multipotent mesenchymal stromal cells derived from different sources, with a focus on neuroregenerative potential

Y Petrenko, I Vackova, K Kekulova, M Chudickova… - Scientific reports, 2020 - nature.com
Multipotent mesenchymal stromal cells (MSCs) can be considered an accessible therapeutic
tool for regenerative medicine. Here, we compared the growth kinetics, immunophenotypic …

Phenotype, donor age and gender affect function of human bone marrow-derived mesenchymal stromal cells

G Siegel, T Kluba, U Hermanutz-Klein, K Bieback… - BMC medicine, 2013 - Springer
Abstract Background Mesenchymal stromal cells (MSCs) are attractive for cell-based
therapies ranging from regenerative medicine and tissue engineering to immunomodulation …

Expansion and characterization of bone marrow derived human mesenchymal stromal cells in serum-free conditions

S Bhat, P Viswanathan, S Chandanala, SJ Prasanna… - Scientific reports, 2021 - nature.com
Bone marrow-derived mesenchymal stromal cells (BM-MSCs) are gaining increasing
importance in the field of regenerative medicine. Although therapeutic value of MSCs is now …

Treatment of acute respiratory distress syndrome with allogeneic adipose-derived mesenchymal stem cells: a randomized, placebo-controlled pilot study

G Zheng, L Huang, H Tong, Q Shu, Y Hu, M Ge… - Respiratory …, 2014 - Springer
Background Recent studies have demonstrated that mesenchymal stem cells (MSCs)
modulate the immune response and reduce lung injury in animal models. Currently, no …

Human platelet lysate to substitute fetal bovine serum in hMSC expansion for translational applications: a systematic review

M Guiotto, W Raffoul, AM Hart, MO Riehle… - Journal of translational …, 2020 - Springer
Background Foetal bovine serum (FBS), is the most commonly used culture medium additive
for in vitro cultures, despite its undefined composition, its potential immunogenicity and …

Evaluation of human platelet lysate versus fetal bovine serum for culture of mesenchymal stromal cells

H Hemeda, B Giebel, W Wagner - Cytotherapy, 2014 - Elsevier
Culture media for therapeutic cell preparations—such as mesenchymal stromal cells (MSCs)—
usually comprise serum additives. Traditionally, fetal bovine serum is supplemented in basic …

Injectable and tunable hyaluronic acid hydrogels releasing chemotactic and angiogenic growth factors for endodontic regeneration

CR Silva, PS Babo, M Gulino, L Costa, JM Oliveira… - Acta biomaterialia, 2018 - Elsevier
Bioengineered soft tissues on any meaningful scale or complexity must incorporate aspects
of the functional tissue, namely a vasculature, providing cells oxygen and nutrients critical for …