For nearly three decades, the sequence of the human mitochondrial genome (mtDNA) has
provided a molecular framework for understanding maternally inherited diseases. However …

Mitochondria serve as a hub for many cellular processes, including bioenergetics,
metabolism, cellular signaling, redox balance, calcium homeostasis, and cell death. The …

Information on protein subcellular localization is important to understand the cellular
functions of proteins. Currently, such information is manually curated from the literature …

Existing protein tagging and detection methods are powerful but have drawbacks. Split
protein tags can perturb protein solubility,,, or may not work in living cells,,. Green …

Mitochondria are tailored to meet the metabolic and signaling needs of each cell. To explore
its molecular composition, we performed a proteomic survey of mitochondria from mouse …

We have studied the naturally split α subunit of the DNA polymerase III (DnaE) intein from
Nostoc punctiforme PCC73102 (Npu) using purified proteins and determined an apparent …

Sarcopenia and exercise intolerance are major contributors to reduced quality of life in the
elderly for which there are few effective treatments. We tested whether enhancing …

We developed genetically encoded RNA probes for characterizing localization and
dynamics of mitochondrial RNA (mtRNA) in single living cells. The probes consist of two …

Protein splicing is a naturally occurring process in which a protein editor, called an intein,
performs a molecular disappearing act by cutting itself out of a host protein in a traceless …

Protein splicing is a naturally occurring process in which an intervening intein domain
excises itself out of a precursor polypeptide in an autocatalytic fashion with concomitant …