Overview of physiological, biochemical, and regulatory aspects of nitrogen fixation in Azotobacter vinelandii

JS Martin del Campo, J Rigsbee… - Critical Reviews in …, 2022 - Taylor & Francis
Understanding how Nature accomplishes the reduction of inert nitrogen gas to form
metabolically tractable ammonia at ambient temperature and pressure has challenged …

Atomic-level design of biomimetic iron-sulfur clusters for biocatalysis

X Zhang, S Zhou, D Liu, K Fan, H Liu - Nanoscale, 2024 - pubs.rsc.org
Designing biomimetic materials with high activity and customized biological functions by
mimicking the central structure of biomolecules has become an important avenue for the …

Heterologous synthesis of the complex homometallic cores of nitrogenase P- and M-clusters in Escherichia coli

R Quechol, JB Solomon, YA Liu… - Proceedings of the …, 2023 - National Acad Sciences
Nitrogenase is an active target of heterologous expression because of its importance for
areas related to agronomy, energy, and environment. One major hurdle for expressing an …

[HTML][HTML] Improvement of the nitrogenase activity in Escherichia coli that expresses the nitrogen fixation-related genes from Azotobacter vinelandii

Y Ito, D Yoshidome, M Hidaka, Y Araki, K Ito… - Biochemical and …, 2024 - Elsevier
The transfer of nitrogen fixation (nif) genes from diazotrophs to non-diazotrophic hosts is of
increasing interest for engineering biological nitrogen fixation. A recombinant Escherichia …

Sequence similarity network and protein structure prediction offer insights into the evolution of microbial pathways for ferrous iron oxidation

L Li, Z Liu, D Meng, Y Liu, T Liu, C Jiang, H Yin - Msystems, 2023 - Am Soc Microbiol
Dissimilatory ferrous iron [Fe (II)] oxidation is a well-established microbial energy generation
strategy. This study aims to comprehensively investigate the distribution and evolution of …

What determines symbiotic nitrogen fixation efficiency in rhizobium: recent insights into Rhizobium leguminosarum

X Li, Z Li - Archives of Microbiology, 2023 - Springer
Symbiotic nitrogen fixation (SNF) by rhizobium, a Gram-negative soil bacterium, is an
essential component in the nitrogen cycle and is a sustainable green way to maintain soil …

Structural correlations of nitrogenase active sites using nuclear resonance vibrational spectroscopy and QM/MM calculations

C Van Stappen, B Benediktsson, A Rana… - Faraday …, 2023 - pubs.rsc.org
The biological conversion of N2 to NH3 is accomplished by the nitrogenase family, which is
collectively comprised of three closely related but unique metalloenzymes. In the present …

Heterologous expression of a fully active Azotobacter vinelandii nitrogenase Fe protein in Escherichia coli

JB Solomon, YA Liu, K Górecki, R Quechol, CC Lee… - Mbio, 2023 - Am Soc Microbiol
The functional versatility of the Fe protein, the reductase component of nitrogenase, makes it
an appealing target for heterologous expression, which could facilitate future …

Understanding the P‐Cluster of Vanadium Nitrogenase: an EPR and XAS Study of the Holo vs. Apo Forms of the Enzyme

IM Wahl, K Sengupta, M van Gastel, L Decamps… - …, 2024 - Wiley Online Library
The catalytic moiety of nitrogenases contains two complex metalloclusters: The M‐cluster
(also called cofactor), where the catalytic reduction of substrates takes place, and the …

[HTML][HTML] Nitrogen fixation by rhizobacterial nif mechanism: an advanced genetic perspective

NI Usman, MM Wali - Updates on Rhizobacteria, 2024 - intechopen.com
The global population's rising nutritional needs pose a challenge, demanding a 70% boost
in agricultural efficiency to feed 10 billion people by 2050. This task is complicated by limited …