Photoinduced chemistry in fluorescent proteins: curse or blessing?

A Acharya, AM Bogdanov, BL Grigorenko… - Chemical …, 2017 - ACS Publications
Photoinduced reactions play an important role in the photocycle of fluorescent proteins from
the green fluorescent protein (GFP) family. Among such processes are photoisomerization …

Fluorescent proteins for live-cell imaging with super-resolution

K Nienhaus, GU Nienhaus - Chemical Society Reviews, 2014 - pubs.rsc.org
Fluorescent proteins (FPs) from the GFP family have become indispensable as marker tools
for imaging live cells, tissues and entire organisms. A wide variety of these proteins have …

Serial femtosecond crystallography reveals that photoactivation in a fluorescent protein proceeds via the hula twist mechanism

A Fadini, CDM Hutchison, D Morozov… - Journal of the …, 2023 - ACS Publications
Chromophore cis/trans photoisomerization is a fundamental process in chemistry and in the
activation of many photosensitive proteins. A major task is understanding the effect of the …

Map** GFP structure evolution during proton transfer with femtosecond Raman spectroscopy

C Fang, RR Frontiera, R Tran, RA Mathies - Nature, 2009 - nature.com
Tracing the transient atomic motions that lie at the heart of chemical reactions requires high-
resolution multidimensional structural information on the timescale of molecular vibrations …

Chromophore twisting in the excited state of a photoswitchable fluorescent protein captured by time-resolved serial femtosecond crystallography

N Coquelle, M Sliwa, J Woodhouse, G Schirò… - Nature Chemistry, 2018 - nature.com
Chromophores absorb light in photosensitive proteins and thereby initiate fundamental
biological processes such as photosynthesis, vision and biofluorescence. An important goal …

Green fluorescent protein: structure, folding and chromophore maturation

TD Craggs - Chemical Society Reviews, 2009 - pubs.rsc.org
The prolific use of green fluorescent protein and its variants throughout cellular biology
relies on the post-translational formation of the chromophore, which proceeds without the …

Electrostatic control of photoisomerization pathways in proteins

MG Romei, CY Lin, II Mathews, SG Boxer - Biophysical Journal, 2020 - cell.com
Adam W. Duster1, Hai Lin2. 1Integrative and Systems Biology, University of Colorado
Denver, Denver, CO, USA, 2Chemistry, University of Colorado Denver, Denver, CO, USA …

Green fluorescent protein inspired fluorophores

J Kong, Y Wang, W Qi, M Huang, R Su, Z He - Advances in Colloid and …, 2020 - Elsevier
Green fluorescence proteins (GFP) are appealing to a variety of biomedical and
biotechnology applications, such as protein fusion, subcellular localizations, cell …

Topochemistry and photomechanical effects in crystals of green fluorescent protein-like chromophores: effects of hydrogen bonding and crystal packing

P Naumov, J Kowalik, KM Solntsev… - Journal of the …, 2010 - ACS Publications
To obtain insight into the effects of the environment on the photophysics and photochemistry
of the green fluorescence protein (GFP), eight crystal structures of six synthetic aryl …

Beta-barrel scaffold of fluorescent proteins: folding, stability and role in chromophore formation

OV Stepanenko, OV Stepanenko, IM Kuznetsova… - International review of …, 2013 - Elsevier
This review focuses on the current view of the interaction between the β-barrel scaffold of
fluorescent proteins and their unique chromophore located in the internal helix. The …