Diversity, classification and evolution of CRISPR-Cas systems
Highlights•Two classes of CRISPR-Cas systems differ by the architectures of effector
modules.•Effectors of Class 2 CRISPR-Cas systems are large, multidomain proteins.•Effector …
modules.•Effectors of Class 2 CRISPR-Cas systems are large, multidomain proteins.•Effector …
Origins and evolution of CRISPR-Cas systems
CRISPR-Cas, the bacterial and archaeal adaptive immunity systems, encompass a complex
machinery that integrates fragments of foreign nucleic acids, mostly from mobile genetic …
machinery that integrates fragments of foreign nucleic acids, mostly from mobile genetic …
C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector
INTRODUCTION Almost all archaea and about half of bacteria possess clustered regularly
interspaced short palindromic repeat (CRISPR)–CRISPR-associated genes (Cas) adaptive …
interspaced short palindromic repeat (CRISPR)–CRISPR-associated genes (Cas) adaptive …
Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection
Bacterial adaptive immune systems use CRISPRs (clustered regularly interspaced short
palindromic repeats) and CRISPR-associated (Cas) proteins for RNA-guided nucleic acid …
palindromic repeats) and CRISPR-associated (Cas) proteins for RNA-guided nucleic acid …
An updated evolutionary classification of CRISPR–Cas systems
KS Makarova, YI Wolf, OS Alkhnbashi… - Nature reviews …, 2015 - nature.com
The evolution of CRISPR–cas loci, which encode adaptive immune systems in archaea and
bacteria, involves rapid changes, in particular numerous rearrangements of the locus …
bacteria, involves rapid changes, in particular numerous rearrangements of the locus …
RNA-targeting CRISPR–Cas systems
CRISPR–Cas is a widespread adaptive immune system in bacteria and archaea that
protects against viral infection by targeting specific invading nucleic acid sequences …
protects against viral infection by targeting specific invading nucleic acid sequences …
Diverse evolutionary roots and mechanistic variations of the CRISPR-Cas systems
BACKGROUND Prokaryotes have evolved multiple systems to combat invaders such as
viruses and plasmids. Examples of such defense systems include receptor masking …
viruses and plasmids. Examples of such defense systems include receptor masking …
Cas13b is a type VI-B CRISPR-associated RNA-guided RNase differentially regulated by accessory proteins Csx27 and Csx28
CRISPR-Cas adaptive immune systems defend microbes against foreign nucleic acids via
RNA-guided endonucleases. Using a computational sequence database mining approach …
RNA-guided endonucleases. Using a computational sequence database mining approach …
Classification and nomenclature of CRISPR-Cas systems: where from here?
As befits an immune mechanism, CRISPR-Cas systems are highly variable with respect to
Cas protein sequences, gene composition, and organization of the genomic loci. Optimal …
Cas protein sequences, gene composition, and organization of the genomic loci. Optimal …
Type III CRISPR–Cas systems produce cyclic oligoadenylate second messengers
In many prokaryotes, type III clustered regularly interspaced short palindromic repeat
(CRISPR)–CRISPR-associated (Cas) systems detect and degrade invasive genetic …
(CRISPR)–CRISPR-associated (Cas) systems detect and degrade invasive genetic …