In-cell structural biology by NMR: the benefits of the atomic scale
FX Theillet - Chemical reviews, 2022 - ACS Publications
In-cell structural biology aims at extracting structural information about proteins or nucleic
acids in their native, cellular environment. This emerging field holds great promise and is …
acids in their native, cellular environment. This emerging field holds great promise and is …
Advances in NMR spectroscopy of weakly aligned biomolecular systems
The measurement and application of residual dipolar couplings (RDCs) in solution NMR
studies of biological macromolecules has become well established over the past quarter of a …
studies of biological macromolecules has become well established over the past quarter of a …
[BOOK][B] NMR of paramagnetic molecules: applications to metallobiomolecules and models
NMR of Paramagnetic Molecules: Applications to Metallobiomolecules and Models, Second
Edition is a self-contained, comprehensive reference for chemists, physicists, and life …
Edition is a self-contained, comprehensive reference for chemists, physicists, and life …
A transient and low-populated protein-folding intermediate at atomic resolution
Proteins can sample conformational states that are critical for function but are seldom
detected directly because of their low occupancies and short lifetimes. In this work, we used …
detected directly because of their low occupancies and short lifetimes. In this work, we used …
NMR approaches for structural analysis of multidomain proteins and complexes in solution
NMR spectroscopy is a key method for studying the structure and dynamics of (large)
multidomain proteins and complexes in solution. It plays a unique role in integrated …
multidomain proteins and complexes in solution. It plays a unique role in integrated …
Intracellular environment can change protein conformational dynamics in cells through weak interactions
M Wang, X Song, J Chen, X Chen, X Zhang, Y Yang… - Science …, 2023 - science.org
Conformational dynamics is important for protein functions, many of which are performed in
cells. How the intracellular environment may affect protein conformational dynamics is …
cells. How the intracellular environment may affect protein conformational dynamics is …
Common sequence motifs of nascent chains engage the ribosome surface and trigger factor
A Deckert, AME Cassaignau, X Wang… - Proceedings of the …, 2021 - National Acad Sciences
In the cell, the conformations of nascent polypeptide chains during translation are modulated
by both the ribosome and its associated molecular chaperone, trigger factor. The specific …
by both the ribosome and its associated molecular chaperone, trigger factor. The specific …
[HTML][HTML] Structural insights into the activity regulation of full-length non-structural protein 1 from SARS-CoV-2
Summary Non-structural protein 1 (Nsp1) of severe acute respiratory syndrome coronavirus
2 (SARS-CoV-2) is a major virulence factor and thus an attractive drug target. The last 33 …
2 (SARS-CoV-2) is a major virulence factor and thus an attractive drug target. The last 33 …
Facile measurement of 1H–15N residual dipolar couplings in larger perdeuterated proteins
We present a simple method, ARTSY, for extracting 1 J NH couplings and 1 H–15 N RDCs
from an interleaved set of two-dimensional 1 H–15 N TROSY-HSQC spectra, based on the …
from an interleaved set of two-dimensional 1 H–15 N TROSY-HSQC spectra, based on the …
Protein folding by NMR
A Zhuravleva, DM Korzhnev - Progress in nuclear magnetic resonance …, 2017 - Elsevier
Protein folding is a highly complex process proceeding through a number of disordered and
partially folded nonnative states with various degrees of structural organization. These …
partially folded nonnative states with various degrees of structural organization. These …