Liquid phase condensation in cell physiology and disease
Y Shin, CP Brangwynne - Science, 2017 - science.org
BACKGROUND Living cells contain distinct subcompartments to facilitate spatiotemporal
regulation of biological reactions. In addition to canonical membrane-bound organelles such …
regulation of biological reactions. In addition to canonical membrane-bound organelles such …
Classification of intrinsically disordered regions and proteins
Over the past decade, we have observed a massive increase in the amount of information
describing protein sequences from a variety of organisms. 1, 2 While this may reflect the …
describing protein sequences from a variety of organisms. 1, 2 While this may reflect the …
The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications
H Götzke, M Kilisch, M Martínez-Carranza… - Nature …, 2019 - nature.com
Specialized epitope tags are widely used for detecting, manipulating or purifying proteins,
but often their versatility is limited. Here, we introduce the ALFA-tag, a rationally designed …
but often their versatility is limited. Here, we introduce the ALFA-tag, a rationally designed …
Introducing protein intrinsic disorder
Proteins are the major component of the living cell. They play crucial roles in the
maintenance of life, and their dysfunctions are known to cause different pathologies. One of …
maintenance of life, and their dysfunctions are known to cause different pathologies. One of …
Hydrophobicity of arginine leads to reentrant liquid-liquid phase separation behaviors of arginine-rich proteins
Intrinsically disordered proteins rich in cationic amino acid groups can undergo Liquid-
Liquid Phase Separation (LLPS) in the presence of charge-balancing anionic counterparts …
Liquid Phase Separation (LLPS) in the presence of charge-balancing anionic counterparts …
The contribution of intrinsically disordered regions to protein function, cellular complexity, and human disease
MM Babu - Biochemical Society Transactions, 2016 - portlandpress.com
In the 1960s, Christian Anfinsen postulated that the unique three-dimensional structure of a
protein is determined by its amino acid sequence. This work laid the foundation for the …
protein is determined by its amino acid sequence. This work laid the foundation for the …
A million peptide motifs for the molecular biologist
A molecular description of functional modules in the cell is the focus of many high-
throughput studies in the postgenomic era. A large portion of biomolecular interactions in …
throughput studies in the postgenomic era. A large portion of biomolecular interactions in …
Rapid prediction and analysis of protein intrinsic disorder
Protein intrinsic disorder is found in all kingdoms of life and is known to underpin numerous
physiological and pathological processes. Computational methods play an important role in …
physiological and pathological processes. Computational methods play an important role in …
Short linear motifs: ubiquitous and functionally diverse protein interaction modules directing cell regulation
The eukaryotic cell is a bustling collection of macromolecules acting cooperatively to
mediate the functions required for cell viability. Specific, context-dependent and tightly …
mediate the functions required for cell viability. Specific, context-dependent and tightly …
Principles of protein structural ensemble determination
Highlights•The principles of protein structural ensemble determination are described.•The
use of experimental data averaged over multiple states is analysed.•Approaches for …
use of experimental data averaged over multiple states is analysed.•Approaches for …