Artificial intelligence in cryo-EM protein particle picking: recent advances and remaining challenges

A Dhakal, R Gyawali, L Wang… - Briefings in …, 2025 - academic.oup.com
Cryo-electron microscopy (cryo-EM) has revolutionized structural biology by enabling the
determination of high-resolution 3-Dimensional (3D) structures of large biological …

Cryo-EM: A window into the dynamic world of RNA molecules

X Zhang, S Li, K Zhang - Current Opinion in Structural Biology, 2024 - Elsevier
RNAs are critical for complex cellular functions, characterized by their structural versatility
and ability to undergo conformational transitions in response to cellular cues. The elusive …

CryoTransformer: a transformer model for picking protein particles from Cryo-EM micrographs

A Dhakal, R Gyawali, L Wang, J Cheng - Bioinformatics, 2024 - academic.oup.com
Motivation Cryo-electron microscopy (cryo-EM) is a powerful technique for determining the
structures of large protein complexes. Picking single protein particles from cryo-EM …

Accurate cryo-EM protein particle picking by integrating the foundational AI image segmentation model and specialized U-Net

R Gyawali, A Dhakal, L Wang, J Cheng - bioRxiv, 2024 - pmc.ncbi.nlm.nih.gov
Picking protein particles in cryo-electron microscopy (cryo-EM) micrographs is a crucial step
in the cryo-EM-based structure determination. However, existing methods trained on a …

Predicting protein-ligand binding structure using E (n) Equivariant graph neural networks

A Dhakal, R Gyawali, J Cheng - bioRxiv, 2023 - biorxiv.org
Drug design is a costly and time-consuming process, often taking more than 12 years and
costing up to billions of dollars. The COVID-19 pandemic has signified the urgent need for …

CryoSegNet: accurate cryo-EM protein particle picking by integrating the foundational AI image segmentation model and attention-gated U-Net

R Gyawali, A Dhakal, L Wang… - Briefings in …, 2024 - academic.oup.com
Picking protein particles in cryo-electron microscopy (cryo-EM) micrographs is a crucial step
in the cryo-EM-based structure determination. However, existing methods trained on a …

DeepLIR: Attention-Based Approach for Mask-Based Lensless Image Reconstruction

A Poudel, U Nakarmi - Proceedings of the IEEE/CVF Winter …, 2024 - openaccess.thecvf.com
Lensless imaging has emerged as a promising solution to overcome the need for expensive
and bulky lenses used in traditional cameras. This technique leverages a mask to optically …

Deep Learning Framework for Inverse Problems in Computational Imaging: A Lensless Imaging and Super-resolution Magnetic Resonance Imaging Case

A Poudel - 2024 - search.proquest.com
Inverse problems in computer vision involve reconstructing an original scene or image from
incomplete, noisy, or indirect measurements. These problems are critical in tasks such as …

A novel particle picking approach for cryo-electron microscopy images

P Zhang, F Zhou - International Conference on Cloud …, 2024 - spiedigitallibrary.org
Cryo-electron microscopy (cryo-EM) has become a crucial tool for determining the structures
of proteins and macromolecular complexes. However, the extremely low signal-to-noise …