Proteins that self-assemble into polyhedral shell-like structures are useful molecular
containers both in nature and in the laboratory. Here we review efforts to repurpose diverse …

The predictable nature of DNA interactions enables the programmable assembly of highly
advanced 2D and 3D DNA structures of nanoscale dimensions. The access to ever larger …

Site-specific protein modification is a widely-used biochemical tool. However, there are
many challenges associated with the development of protein modification techniques, in …

This review provides an overview of recent developments in “chemical virology.” Viruses, as
materials, provide unique nanoscale scaffolds that have relevance in chemical biology and …

This protocol describes the use of sortase-mediated reactions to label the N terminus of any
given protein of interest. The sortase recognition sequence, LPXTG (for Staphylococcus …

Highlights•Sortase-catalyzed ligation is a powerful strategy for protein modification.•
Engineered sortases improve reaction rates and eliminate Ca 2+ dependency.•Deactivation …

Molecular biologists and chemists alike have long sought to modify proteins with
substituents that cannot be installed by standard or even advanced genetic approaches. We …

Sortase‐mediated ligation (SML) has emerged as a powerful and versatile methodology for
site‐specific protein conjugation, functionalization/labeling, immobilization, and design of …

Proteins and DNA are two commonly used molecules for self-assembling nanotechnology.
In this tutorial review, we discuss the hybrid field of" protein-DNA nanotechnology," whereby …

Creation of functional protein bioconjugates demands methods for attaching a diverse array
of probes to target proteins with high specificity, under mild conditions. The sortase A …