Clinical Diagnostics of Bacterial Infections and Their Resistance to Antibiotics—Current State and Whole Genome Sequencing Implementation Perspectives

E Avershina, A Khezri, R Ahmad - Antibiotics, 2023 - mdpi.com
Antimicrobial resistance (AMR), defined as the ability of microorganisms to withstand
antimicrobial treatment, is responsible for millions of deaths annually. The rapid spread of …

The notable achievements and the prospects of bacterial pathogen genomics

GD Amoutzias, M Nikolaidis, A Hesketh - Microorganisms, 2022 - mdpi.com
Throughout the entirety of human history, bacterial pathogens have played an important role
and even shaped the fate of civilizations. The application of genomics within the last 27 …

A rapid bacterial pathogen and antimicrobial resistance diagnosis workflow using Oxford nanopore adaptive sequencing method

H Cheng, Y Sun, Q Yang, M Deng, Z Yu… - Briefings in …, 2022 - academic.oup.com
Metagenomic sequencing analysis (mNGS) has been implemented as an alternative
approach for pathogen diagnosis in recent years, which is independent of cultivation and is …

An integrated method for targeted Oxford Nanopore sequencing and automated bioinformatics for the simultaneous detection of bacteria, fungi, and ARG

S Kuruwa, A Zade, S Shah, R Moidu… - Journal of Applied …, 2024 - academic.oup.com
Aims The use of metagenomics for pathogen identification in clinical practice has been
limited. Here we describe a workflow to encourage the clinical utility and potential of NGS for …

The application of targeted nanopore sequencing for the identification of pathogens and resistance genes in lower respiratory tract infections

H Zhang, M Wang, X Han, T Wang, Y Lei… - Frontiers in …, 2022 - frontiersin.org
Objectives Lower respiratory tract infections (LRTIs) are one of the causes of mortality
among infectious diseases. Microbial cultures commonly used in clinical practice are time …

Hospital antimicrobial stewardship: profiling the oral microbiome after exposure to COVID-19 and antibiotics

P Buendia, K Fernandez, C Raley… - Frontiers in …, 2024 - frontiersin.org
Introduction During the COVID-19 Delta variant surge, the CLAIRE cross-sectional study
sampled saliva from 120 hospitalized patients, 116 of whom had a positive COVID-19 PCR …

Optimizing nanopore sequencing for rapid detection of microbial species and antimicrobial resistance in patients at risk of surgical site infections

E Whittle, JA Yonkus, P Jeraldo, R Alva-Ruiz, H Nelson… - Msphere, 2022 - Am Soc Microbiol
Surgical site infections (SSI) are a significant burden to patients and health care systems.
We evaluated the use of Nanopore sequencing (NS) to rapidly detect microbial species and …

Rapid detection of multidrug resistance in tuberculosis using nanopore-based targeted next-generation sequencing: a multicenter, double-blind study

A Liu, S Liu, K Lv, Q Zhu, J Wen, J Li, C Liang… - Frontiers in …, 2024 - frontiersin.org
Background Resistance to anti-tuberculous drugs is a major challenge in the treatment of
tuberculosis (TB). We aimed to evaluate the clinical availability of nanopore-based targeted …

An ultra-sensitive bacterial pathogen and antimicrobial resistance diagnosis workflow using Oxford Nanopore adaptive sampling sequencing method

H Cheng, Y Sun, Q Yang, M Deng, Z Yu, L Liu, L Yang… - medRxiv, 2022 - medrxiv.org
Metagenomic sequencing analysis has been implemented as an alternative approach for
pathogen diagnosis in recent years, which is independent on cultivation and able to identify …

Unlocking the Power of Nanopores: Recent Advances in Biosensing Applications and Analog Front-End

M Liu, J Li, CS Tan - Biosensors, 2023 - mdpi.com
The biomedical field has always fostered innovation and the development of various new
technologies. Beginning in the last century, demand for picoampere-level current detection …