Single-cell omics: experimental workflow, data analyses and applications

F Sun, H Li, D Sun, S Fu, L Gu, X Shao, Q Wang… - Science China Life …, 2024‏ - Springer
Cells are the fundamental units of biological systems and exhibit unique development
trajectories and molecular features. Our exploration of how the genomes orchestrate the …

Applications of single-cell technologies in drug discovery for tumor treatment

B Liu, S Hu, X Wang - Iscience, 2024‏ - cell.com
Single-cell technologies have been known as advanced and powerful tools to study tumor
biological systems at the single-cell resolution and are playing increasingly critical roles in …

Luminal breast epithelial cells of BRCA1 or BRCA2 mutation carriers and noncarriers harbor common breast cancer copy number alterations

MJ Williams, MUJ Oliphant, V Au, C Liu, C Baril… - Nature Genetics, 2024‏ - nature.com
The prevalence and nature of somatic copy number alterations (CNAs) in breast epithelium
and their role in tumor initiation and evolution remain poorly understood. Using single-cell …

Normal breast tissues harbour rare populations of aneuploid epithelial cells

Y Lin, J Wang, K Wang, S Bai, A Thennavan, R Wei… - Nature, 2024‏ - nature.com
Aneuploid epithelial cells are common in breast cancer,; however, their presence in normal
breast tissues is not well understood. To address this question, we applied single-cell DNA …

Progression from ductal carcinoma in situ to invasive breast cancer: molecular features and clinical significance

J Wang, B Li, M Luo, J Huang, K Zhang… - … and Targeted Therapy, 2024‏ - nature.com
Ductal carcinoma in situ (DCIS) represents pre-invasive breast carcinoma. In untreated
cases, 25–60% DCIS progress to invasive ductal carcinoma (IDC). The challenge lies in …

Single Nucleus Total RNA Sequencing of Formalin‐Fixed Paraffin‐Embedded Gliomas

Z Xu, L Chen, X Lin, Y Lyu, M Zhou, H Chen… - Small …, 2024‏ - Wiley Online Library
Gliomas, the predominant form of brain cancer, comprise diverse malignant subtypes with
limited curative therapies available. The insufficient understanding of their molecular …

High clonal diversity and spatial genetic admixture in early prostate cancer and surrounding normal tissue

N Zhang, L Harbers, M Simonetti, C Diekmann… - Nature …, 2024‏ - nature.com
Somatic copy number alterations (SCNAs) are pervasive in advanced human cancers, but
their prevalence and spatial distribution in early-stage, localized tumors and their …

Injectable nano-in situ-thermosensitive-hydrogels based on halofuginone and silver for postoperative treatment against triple-negative breast cancer

R Zuo, J Gong, X Gao, E Nepovimova, J Zhang… - International Journal of …, 2024‏ - Elsevier
Postoperative distant metastasis and high recurrence rate causes a dilemma in treating
triple-negative breast cancer (TNBC) owing to its unforeseeable invasion into various …

Serial single-cell RNA sequencing unveils drug resistance and metastatic traits in stage IV breast cancer

K Otsuji, Y Takahashi, T Osako, T Kobayashi… - NPJ Precision …, 2024‏ - nature.com
Metastasis is a complex process that remains poorly understood at the molecular levels. We
profiled single-cell transcriptomic, genomic, and epigenomic changes associated with …

Conquering Overtreatment of DCIS: Lessons from PRECISION

P Bhattacharjee, EH Lips, EJ Sawyer, ES Hwang… - Cancer …, 2025‏ - aacrjournals.org
As we cannot reliably distinguish indolent, low-risk ductal carcinoma in situ (DCIS) from
potentially progressive, high-risk DCIS, all women with DCIS diagnosis undergo intensive …