Highlights•The number of structures determined by cryo-EM is continuing to grow
rapidly.•Computational methods are hel** resolve conformational heterogeneity.• …

The recent revolution in cryo-EM has produced an explosion of structures at near-atomic or
better resolution. This has allowed cryo-EM structures to provide visualization of bound …

Recent advances in single-particle cryoelecton microscopy (cryo-EM) are enabling
generation of numerous near-atomic resolution structures for well-ordered protein …

We report a fully automated procedure for the optimization and interpretation of
reconstructions from cryo-electron microscopy (cryo-EM) data, available in Phenix as …

Reversible post-translational modifications represent a mechanism to control tumor
metabolism. Here we show that mitochondrial Sirtuin5 (SIRT5), which mediates lysine …

Plant metabolism is finely orchestrated to allow the occurrence of complementary and
sometimes opposite metabolic pathways. In part this is achieved by the allosteric regulation …

In the past two decades, drug candidates with a covalent binding mode have gained the
interest of medicinal chemists, as several covalent anticancer drugs have successfully …

Increasing attention has been focused on the study of protein–metabolite interactions (PMI),
which play a key role in regulating protein functions and directing an orchestra of cellular …

Highlights•Studying biomolecular folding with optical tweezers grants high dynamic
range.•Combining optical tweezers with molecular dynamics simulation enables accurate …

Atomic structures of adeno-associated virus (AAV)-DJ, alone and in complex with
fondaparinux, have been determined by cryoelectron microscopy at 3 Å resolution. The …