The determination of HIV-1 RT mutation rate, its possible allosteric effects, and its implications on drug resistance
The high mutation rate of the human immunodeficiency virus type 1 (HIV-1) plays a major
role in treatment resistance, from the development of vaccines to therapeutic drugs. In …
role in treatment resistance, from the development of vaccines to therapeutic drugs. In …
[HTML][HTML] Mutation rates and intrinsic fidelity of retroviral reverse transcriptases
L Menéndez-Arias - Viruses, 2009 - mdpi.com
Retroviruses are RNA viruses that replicate through a DNA intermediate, in a process
catalyzed by the viral reverse transcriptase (RT). Although cellular polymerases and host …
catalyzed by the viral reverse transcriptase (RT). Although cellular polymerases and host …
Analysis of CLIP and iCLIP methods for nucleotide-resolution studies of protein-RNA interactions
UV cross-linking and immunoprecipitation (CLIP) and individual-nucleotide resolution CLIP
(iCLIP) are methods to study protein-RNA interactions in untreated cells and tissues. Here …
(iCLIP) are methods to study protein-RNA interactions in untreated cells and tissues. Here …
Mutations in HIV-1 reverse transcriptase affect the errors made in a single cycle of viral replication
The genetic variation in HIV-1 in patients is due to the high rate of viral replication, the high
viral load, and the errors made during viral replication. Some of the mutations in reverse …
viral load, and the errors made during viral replication. Some of the mutations in reverse …
[HTML][HTML] Structural aspects of drug resistance and inhibition of HIV-1 reverse transcriptase
HIV-1 Reverse Transcriptase (HIV-1 RT) has been the target of numerous approved anti-
AIDS drugs that are key components of Highly Active Anti-Retroviral Therapies (HAART). It …
AIDS drugs that are key components of Highly Active Anti-Retroviral Therapies (HAART). It …
In vivo and in vitro intragenomic rearrangement of TT viruses
L Leppik, K Gunst, M Lehtinen, J Dillner… - Journal of …, 2007 - Am Soc Microbiol
The in vitro replication of the Torque teno virus (TT virus) tth8 full-length genome and particle
formation in a Hodgkin's lymphoma-derived cell line after transfection with cloned viral DNA …
formation in a Hodgkin's lymphoma-derived cell line after transfection with cloned viral DNA …
[HTML][HTML] In vitro fidelity of the prototype primate foamy virus (PFV) RT compared to HIV-1 RT
PL Boyer, CR Stenbak, D Hoberman, ML Linial… - Virology, 2007 - Elsevier
We compared the in vitro fidelity of wild-type human immunodeficiency virus type-1 (HIV-1)
reverse transcriptase (RT) and the prototype foamy virus (PFV) RT. Both enzymes had …
reverse transcriptase (RT) and the prototype foamy virus (PFV) RT. Both enzymes had …
Altered error specificity of RNase H-deficient HIV-1 reverse transcriptases during DNA-dependent DNA synthesis
M Alvarez, V Barrioluengo… - Nucleic acids …, 2013 - academic.oup.com
Asp 443 and Glu 478 are essential active site residues in the RNase H domain of human
immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT). We have investigated the …
immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT). We have investigated the …
Transcript slippage and recoding
Accurate transmission of genetic information during transcription requires that RNA
polymerases maintain the correct register of the active site during each cycle of nucleotide …
polymerases maintain the correct register of the active site during each cycle of nucleotide …
Mechanistic insights into the role of Val75 of HIV-1 reverse transcriptase in misinsertion and mispair extension fidelity of DNA synthesis
T Matamoros, B Kim, L Menéndez-Arias - Journal of molecular biology, 2008 - Elsevier
The side chain of Val75 stabilizes the fingers subdomain of the human immunodeficiency
virus type 1 reverse transcriptase (RT), while its peptide backbone interacts with the single …
virus type 1 reverse transcriptase (RT), while its peptide backbone interacts with the single …