Glycoproteomics

I Bagdonaite, SA Malaker, DA Polasky… - Nature Reviews …, 2022 - nature.com
Protein glycosylation involves the co-translational or post-translational addition of glycans to
proteins and is a crucial protein modification in health and disease. The aim of …

A pragmatic guide to enrichment strategies for mass spectrometry–based glycoproteomics

NM Riley, CR Bertozzi, SJ Pitteri - Molecular & Cellular Proteomics, 2021 - ASBMB
Glycosylation is a prevalent, yet heterogeneous modification with a broad range of
implications in molecular biology. This heterogeneity precludes enrichment strategies that …

Precise, fast and comprehensive analysis of intact glycopeptides and modified glycans with pGlyco3

WF Zeng, WQ Cao, MQ Liu, SM He, PY Yang - Nature Methods, 2021 - nature.com
Great advances have been made in mass spectrometric data interpretation for intact
glycopeptide analysis. However, accurate identification of intact glycopeptides and modified …

Optimal Dissociation Methods Differ for N- and O-Glycopeptides

NM Riley, SA Malaker, MD Driessen… - Journal of proteome …, 2020 - ACS Publications
Site-specific characterization of glycosylation requires intact glycopeptide analysis, and
recent efforts have focused on how to best interrogate glycopeptides using tandem mass …

The human disease gene LYSET is essential for lysosomal enzyme transport and viral infection

CM Richards, S Jabs, W Qiao, LD Varanese… - Science, 2022 - science.org
Lysosomes are key degradative compartments of the cell. Transport to lysosomes relies on
GlcNAc-1-phosphotransferase–mediated tagging of soluble enzymes with mannose 6 …

Meta-heterogeneity: evaluating and describing the diversity in glycosylation between sites on the same glycoprotein

T Čaval, AJR Heck, KR Reiding - Molecular & Cellular Proteomics, 2021 - ASBMB
Mass spectrometry–based glycoproteomics has gone through some incredible
developments over the last few years. Technological advances in glycopeptide enrichment …

[HTML][HTML] Roles of glycosylation at the cancer cell surface: opportunities for large scale glycoproteomics

T Čaval, F Alisson-Silva, F Schwarz - Theranostics, 2023 - ncbi.nlm.nih.gov
Cell surface glycosylation has a variety of functions, and its dysregulation in cancer
contributes to impaired signaling, metastasis and the evasion of the immune responses …

Recent advances in software tools for more generic and precise intact glycopeptide analysis

W Cao, M Liu, S Kong, M Wu, Y Zhang… - Molecular & Cellular …, 2021 - ASBMB
Intact glycopeptide identification has long been known as a key and challenging barrier to
the comprehensive and accurate understanding the role of glycosylation in an organism …

The Hitchhiker's guide to glycoproteomics

T Oliveira, M Thaysen-Andersen… - Biochemical Society …, 2021 - portlandpress.com
Protein glycosylation is one of the most common post-translational modifications that are
essential for cell function across all domains of life. Changes in glycosylation are considered …

Comprehensive analysis of the glycan complement of SARS-CoV-2 spike proteins using signature ions-triggered electron-transfer/higher-energy collisional …

D Wang, J Baudys, JL Bundy, M Solano… - Analytical …, 2020 - ACS Publications
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to a global
pandemic of coronavirus disease 2019 (COVID-19). The spike protein expressed on the …