How cryo‐electron microscopy and X‐ray crystallography complement each other

HW Wang, JW Wang - Protein Science, 2017 - Wiley Online Library
With the ability to resolve structures of macromolecules at atomic resolution, X‐ray
crystallography has been the most powerful tool in modern structural biology. At the same …

The CryoEM method MicroED as a powerful tool for small molecule structure determination

CG Jones, MW Martynowycz, J Hattne… - ACS Central …, 2018 - ACS Publications
In the many scientific endeavors that are driven by organic chemistry, unambiguous
identification of small molecules is of paramount importance. Over the past 50 years, NMR …

Current trends and advancements in crystallization and single-crystal structural analysis of small molecules

Y **ao, C Wu, L Zhou, P Commins, L Li… - Coordination Chemistry …, 2024 - Elsevier
The advancement of single-crystal structural analysis has emerged as a pivotal technology
surpassing spectroscopic methods in revealing the intricate structural details of organic …

Structure of the toxic core of α-synuclein from invisible crystals

JA Rodriguez, MI Ivanova, MR Sawaya, D Cascio… - Nature, 2015 - nature.com
The protein α-synuclein is the main component of Lewy bodies, the neuron-associated
aggregates seen in Parkinson disease and other neurodegenerative pathologies. An 11 …

[PDF][PDF] MicroED data collection and processing

J Hattne, FE Reyes, BL Nannenga, D Shi… - … A: Foundations and …, 2015 - journals.iucr.org
MicroED, a method at the intersection of X-ray crystallography and electron cryo-microscopy,
has rapidly progressed by exploiting advances in both fields and has already been …

Ab initio structure determination from prion nanocrystals at atomic resolution by MicroED

MR Sawaya, J Rodriguez, D Cascio… - Proceedings of the …, 2016 - National Acad Sciences
Electrons, because of their strong interaction with matter, produce high-resolution diffraction
patterns from tiny 3D crystals only a few hundred nanometers thick in a frozen-hydrated …

Current trends in protein crystallization

JA Gavira - Archives of biochemistry and biophysics, 2016 - Elsevier
Proteins belong to the most complex colloidal system in terms of their physicochemical
properties, size and conformational-flexibility. This complexity contributes to their great …

Structure of amyloid-β (20-34) with Alzheimer's-associated isomerization at Asp23 reveals a distinct protofilament interface

RA Warmack, DR Boyer, CT Zee, LS Richards… - Nature …, 2019 - nature.com
Amyloid-β (Aβ) harbors numerous posttranslational modifications (PTMs) that may affect
Alzheimer's disease (AD) pathogenesis. Here we present the 1.1 Å resolution MicroED …

[PDF][PDF] Automated electron diffraction tomography–development and applications

U Kolb, Y Krysiak, S Plana-Ruiz - Acta Crystallographica Section B …, 2019 - journals.iucr.org
Electron diffraction tomography (EDT) has gained increasing interest, starting with the
development of automated electron diffraction tomography (ADT) which enables the …

On the quality of the continuous rotation electron diffraction data for accurate atomic structure determination of inorganic compounds

Y Wang, T Yang, H Xu, X Zou… - Journal of Applied …, 2018 - journals.iucr.org
The continuous rotation electron diffraction (cRED) method has the capability of providing
fast three-dimensional electron diffraction data collection on existing and future transmission …