Evolutionary pathways and trajectories in antibiotic resistance

F Baquero, JL Martinez, V F. Lanza… - Clinical Microbiology …, 2021 - Am Soc Microbiol
Evolution is the hallmark of life. Descriptions of the evolution of microorganisms have
provided a wealth of information, but knowledge regarding “what happened” has precluded …

Quantifying and understanding the fitness effects of protein mutations: Laboratory versus nature

JI Boucher, DNA Bolon, DS Tawfik - Protein Science, 2016 - Wiley Online Library
The last decade has seen a growing number of experiments aimed at systematically
map** the effects of mutations in different proteins, and of attempting to correlate their …

Accurate measurement of the effects of all amino-acid mutations on influenza hemagglutinin

MB Doud, JD Bloom - Viruses, 2016 - mdpi.com
Influenza genes evolve mostly via point mutations, and so knowing the effect of every amino-
acid mutation provides information about evolutionary paths available to the virus. We and …

Inference of the distribution of selection coefficients for new nonsynonymous mutations using large samples

BY Kim, CD Huber, KE Lohmueller - Genetics, 2017 - academic.oup.com
The distribution of fitness effects (DFE) has considerable importance in population genetics.
To date, estimates of the DFE come from studies using a small number of individuals. Thus …

High‐throughput discovery of functional disordered regions: investigation of transactivation domains

CNJ Ravarani, TY Erkina, G De Baets… - Molecular Systems …, 2018 - embopress.org
Over 40% of proteins in any eukaryotic genome encode intrinsically disordered regions (IDR
s) that do not adopt defined tertiary structures. Certain IDR s perform critical functions, but …

Deconstruction of the Ras switching cycle through saturation mutagenesis

P Bandaru, NH Shah, M Bhattacharyya, JP Barton… - Elife, 2017 - elifesciences.org
Ras proteins are highly conserved signaling molecules that exhibit regulated, nucleotide-
dependent switching between active and inactive states. The high conservation of Ras …

Systematic mutant analyses elucidate general and client-specific aspects of Hsp90 function

P Mishra, JM Flynn, TN Starr, DNA Bolon - Cell reports, 2016 - cell.com
To probe the mechanism of the Hsp90 chaperone that is required for the maturation of many
signaling proteins in eukaryotes, we analyzed the effects of all individual amino acid …

Comprehensive fitness maps of Hsp90 show widespread environmental dependence

JM Flynn, A Rossouw, P Cote-Hammarlof, I Fragata… - Elife, 2020 - elifesciences.org
Gene-environment interactions have long been theorized to influence molecular evolution.
However, the environmental dependence of most mutations remains unknown. Using deep …

Experimental estimation of the effects of all amino-acid mutations to HIV's envelope protein on viral replication in cell culture

HK Haddox, AS Dingens, JD Bloom - PLoS pathogens, 2016 - journals.plos.org
HIV is notorious for its capacity to evade immunity and anti-viral drugs through rapid
sequence evolution. Knowledge of the functional effects of mutations to HIV is critical for …

Systematic map** of protein mutational space by prolonged drift reveals the deleterious effects of seemingly neutral mutations

L Rockah-Shmuel, Á Tóth-Petróczy… - PLoS computational …, 2015 - journals.plos.org
Systematic map**s of the effects of protein mutations are becoming increasingly popular.
Unexpectedly, these experiments often find that proteins are tolerant to most amino acid …