3D cell culture systems: tumor application, advantages, and disadvantages

O Habanjar, M Diab-Assaf, F Caldefie-Chezet… - International journal of …, 2021 - mdpi.com
The traditional two-dimensional (2D) in vitro cell culture system (on a flat support) has long
been used in cancer research. However, this system cannot be fully translated into clinical …

Three-Dimensional in Vitro Cell Culture Models in Drug Discovery and Drug Repositioning

SA Langhans - Frontiers in pharmacology, 2018 - frontiersin.org
Drug development is a lengthy and costly process that proceeds through several stages
from target identification to lead discovery and optimization, preclinical validation and …

Enhanced substrate stress relaxation promotes filopodia-mediated cell migration

K Adebowale, Z Gong, JC Hou, KM Wisdom… - Nature materials, 2021 - nature.com
Cell migration on two-dimensional substrates is typically characterized by lamellipodia at the
leading edge, mature focal adhesions and spread morphologies. These observations result …

Comparison of 2D and 3D cell culture models for cell growth, gene expression and drug resistance

JC Fontoura, C Viezzer, FG Dos Santos… - Materials Science and …, 2020 - Elsevier
In vitro drug screening is widely used in the development of new drugs, because they
constitute a cost-effective approach to select compounds with more potential for therapy …

Matrix mechanical plasticity regulates cancer cell migration through confining microenvironments

KM Wisdom, K Adebowale, J Chang, JY Lee… - Nature …, 2018 - nature.com
Studies of cancer cell migration have found two modes: one that is protease-independent,
requiring micron-sized pores or channels for cells to squeeze through, and one that is …

Biophysics in tumor growth and progression: from single mechano-sensitive molecules to mechanomedicine

Y **n, K Li, M Huang, C Liang, D Siemann, L Wu, Y Tan… - Oncogene, 2023 - nature.com
Evidence from physical sciences in oncology increasingly suggests that the interplay
between the biophysical tumor microenvironment and genetic regulation has significant …

[HTML][HTML] Confinement and low adhesion induce fast amoeboid migration of slow mesenchymal cells

YJ Liu, M Le Berre, F Lautenschlaeger, P Maiuri… - Cell, 2015 - cell.com
The mesenchymal-amoeboid transition (MAT) was proposed as a mechanism for cancer
cells to adapt their migration mode to their environment. While the molecular pathways …

Steering cell migration: lamellipodium dynamics and the regulation of directional persistence

M Krause, A Gautreau - Nature reviews Molecular cell biology, 2014 - nature.com
Membrane protrusions at the leading edge of cells, known as lamellipodia, drive cell
migration in many normal and pathological situations. Lamellipodial protrusion is powered …

[HTML][HTML] Cancer invasion and the microenvironment: plasticity and reciprocity

P Friedl, S Alexander - Cell, 2011 - cell.com
Cancer invasion is a cell-and tissue-driven process for which the physical, cellular, and
molecular determinants adapt and react throughout the progression of the disease. Cancer …

The matrix environmental and cell mechanical properties regulate cell migration and contribute to the invasive phenotype of cancer cells

CT Mierke - Reports on Progress in Physics, 2019 - iopscience.iop.org
The minimal structural unit of a solid tumor is a single cell or a cellular compartment such as
the nucleus. A closer look inside the cells reveals that there are functional compartments or …