Primer design for quantitative real-time PCR for the emerging Coronavirus SARS-CoV-2

D Li, J Zhang, J Li - Theranostics, 2020 - pmc.ncbi.nlm.nih.gov
In December 2019, a new coronavirus disease (COVID-19) outbreak occurred in Wuhan,
China. Severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2), which is the …

Fast and reliable molecular methods to detect fungal pathogens in woody plants

N Luchi, R Ioos, A Santini - Applied microbiology and biotechnology, 2020 - Springer
Plant diseases caused by pathogenic microorganisms represent a serious threat to plant
productivity, food security, and natural ecosystems. An effective framework for early warning …

RT-qPCR testing of SARS-CoV-2: a primer

SA Bustin, T Nolan - International journal of molecular sciences, 2020 - mdpi.com
Testing for the presence of coronavirus is an essential diagnostic tool for monitoring and
managing the current COVID-19 pandemic. The only reliable test in current use for testing …

The digital MIQE guidelines update: minimum information for publication of quantitative digital PCR experiments for 2020

JF Huggett - Clinical chemistry, 2020 - academic.oup.com
Digital PCR (dPCR) has developed considerably since the publication of the Minimum
Information for Publication of Digital PCR Experiments (dMIQE) guidelines in 2013, with …

The need for robust qPCR‐based eDNA detection assays in environmental monitoring and species inventories

VS Langlois, MJ Allison, LC Bergman, TA To… - Environmental …, 2021 - Wiley Online Library
Considerable promise and excitement exist in the application of environmental DNA (eDNA)
methods to environmental monitoring and species inventories as eDNA can provide cost …

Primer set 2.0 for highly parallel qPCR array targeting antibiotic resistance genes and mobile genetic elements

RD Stedtfeld, X Guo, TM Stedtfeld… - FEMS microbiology …, 2018 - academic.oup.com
The high-throughput antibiotic resistance gene (ARG) qPCR array, initially published in
2012, is increasingly used to quantify resistance and mobile determinants in environmental …

Development and evaluation of fish eDNA metabarcoding assays facilitate the detection of cryptic seahorse taxa (family: Syngnathidae)

GM Nester, M De Brauwer, A Koziol… - Environmental …, 2020 - Wiley Online Library
Environmental DNA (eDNA) metabarcoding methods have demonstrated their potential as
noninvasive techniques for the monitoring and conservation of marine fishes, including rare …

Epidemiological investigation and genetic evolutionary analysis of PRRSV-1 on a pig farm in China

C Li, H Xu, J Zhao, B Gong, Q Sun, L **ang… - Frontiers in …, 2022 - frontiersin.org
Porcine reproductive and respiratory syndrome virus (PRRSV) has brought serious
economic losses to pig industry. PRRSV-1 have existed in China for more than 25 years …

2020 White paper on recent issues in bioanalysis: Vaccine assay validation, qPCR assay validation, QC for CAR-T flow cytometry, NAb assay harmonization and …

B Corsaro, T Yang, R Murphy, I Sonderegger, A Exley… - Bioanalysis, 2021 - Taylor & Francis
The 14th edition of the Workshop on Recent Issues in Bioanalysis (14th WRIB) was held
virtually on June 15-29, 2020 with an attendance of over 1000 representatives from …

Detection of Toxoplasma gondii oocysts in fresh vegetables and berry fruits

CS Marques, S Sousa, A Castro, JMC da Costa - Parasites & vectors, 2020 - Springer
Background Toxoplasma gondii is the third most important contributor to health burden
caused by food-borne illness. Ingestion of tissue cysts from undercooked meat is an …