Human organoids: New strategies and methods for analyzing human development and disease

NS Corsini, JA Knoblich - Cell, 2022 - cell.com
For decades, insight into fundamental principles of human biology and disease has been
obtained primarily by experiments in animal models. While this has allowed researchers to …

Unraveling mechanisms of human brain evolution

MA Lancaster - Cell, 2024 - cell.com
Evolutionary changes in human brain structure and function have enabled our specialized
cognitive abilities. How these changes have come about genetically and functionally has …

Neuroinvasion of SARS-CoV-2 in human and mouse brain

E Song, C Zhang, B Israelow, A Lu-Culligan… - Journal of Experimental …, 2021 - rupress.org
Although COVID-19 is considered to be primarily a respiratory disease, SARS-CoV-2 affects
multiple organ systems including the central nervous system (CNS). Yet, there is no …

Generating human neural diversity with a multiplexed morphogen screen in organoids

ND Amin, KW Kelley, K Kaganovsky, M Onesto, J Hao… - Cell Stem Cell, 2024 - cell.com
Morphogens choreograph the generation of remarkable cellular diversity in the develo**
nervous system. Differentiation of stem cells in vitro often relies upon the combinatorial …

Choroid plexus mast cells drive tumor-associated hydrocephalus

Y Li, C Di, S Song, Y Zhang, Y Lu, J Liao, B Lei… - Cell, 2023 - cell.com
Tumor-associated hydrocephalus (TAH) is a common and lethal complication of brain
metastases. Although other factors beyond mechanical obstructions have been suggested …

[HTML][HTML] A review of protocols for brain organoids and applications for disease modeling

CN Mayhew, R Singhania - STAR protocols, 2023 - Elsevier
Recent breakthroughs in human stem cell technologies have enabled the generation of 3D
brain organoid platforms for modeling human neurodevelopment and disease. Here, we …

Human assembloids reveal the consequences of CACNA1G gene variants in the thalamocortical pathway

J Kim, Y Miura, MY Li, O Revah, S Selvaraj, F Birey… - Neuron, 2024 - cell.com
Abnormalities in thalamocortical crosstalk can lead to neuropsychiatric disorders. Variants in
CACNA1G, which encodes the α1G subunit of the thalamus-enriched T-type calcium …

High-resolution transcriptional landscape of xeno-free human induced pluripotent stem cell-derived cerebellar organoids

S Nayler, D Agarwal, F Curion, R Bowden… - Scientific Reports, 2021 - nature.com
Current protocols for producing cerebellar neurons from human pluripotent stem cells
(hPSCs) often rely on animal co-culture and mostly exist as monolayers, limiting their …

Volumetric compression by heterogeneous scaffold embedding promotes cerebral organoid maturation and does not impede growth

X Tang, Z Wang, D Khutsishvili, Y Cheng, J Wang… - Cell Systems, 2023 - cell.com
Although biochemical regulation has been extensively studied in organoid modeling
protocols, the role of mechanoregulation in directing stem cell fate and organoid …

Advances in 3D neuronal microphysiological systems: towards a functional nervous system on a chip

WA Anderson, A Bosak, HT Hogberg, T Hartung… - In Vitro Cellular & …, 2021 - Springer
Microphysiological systems (MPS) designed to study the complexities of the peripheral and
central nervous systems have made marked improvements over the years and have allowed …