Isobaric labeling-based relative quantification in shotgun proteomics

N Rauniyar, JR Yates III - Journal of proteome research, 2014 - ACS Publications
Mass spectrometry plays a key role in relative quantitative comparisons of proteins in order
to understand their functional role in biological systems upon perturbation. In this review, we …

Multi-dimensional liquid chromatography in proteomics—a review

X Zhang, A Fang, CP Riley, M Wang, FE Regnier… - Analytica chimica …, 2010 - Elsevier
Proteomics is the large-scale study of proteins, particularly their expression, structures and
functions. This still-emerging combination of technologies aims to describe and characterize …

Simultaneous analysis of relative protein expression levels across multiple samples using iTRAQ isobaric tags with 2D nano LC–MS/MS

RD Unwin, JR Griffiths, AD Whetton - Nature protocols, 2010 - nature.com
In this paper, we describe the use of iTRAQ (isobaric Tags for Relative and Absolute
Quantitation) tags for comparison of protein expression levels between multiple samples …

RNA editing of Filamin A pre‐mRNA regulates vascular contraction and diastolic blood pressure

M Jain, TD Mann, M Stulić, SP Rao, A Kirsch… - The EMBO …, 2018 - embopress.org
Epitranscriptomic events such as adenosine‐to‐inosine (A‐to‐I) RNA editing by ADAR can
recode mRNA s to translate novel proteins. Editing of the mRNA that encodes actin …

Breast cancer classification based on proteotypes obtained by SWATH mass spectrometry

P Bouchal, OT Schubert, J Faktor, L Capkova… - Cell reports, 2019 - cell.com
Accurate classification of breast tumors is vital for patient management decisions and
enables more precise cancer treatment. Here, we present a quantitative proteoty** …

A Model of Aerobic and Anaerobic Metabolism of Hydrogen in the Extremophile Acidithiobacillus ferrooxidans

J Kucera, J Lochman, P Bouchal, E Pakostova… - Frontiers in …, 2020 - frontiersin.org
Hydrogen can serve as an electron donor for chemolithotrophic acidophiles, especially in
the deep terrestrial subsurface and geothermal ecosystems. Nevertheless, the current …

Desmocollin-1 is associated with pro-metastatic phenotype of luminal A breast cancer cells and is modulated by parthenolide

P Lapcik, P Sulc, L Janacova, K Jilkova… - Cellular & Molecular …, 2023 - Springer
Abstract Background Desmocollin-1 (DSC1) is a desmosomal transmembrane glycoprotein
that maintains cell-to-cell adhesion. DSC1 was previously associated with lymph node …

Discovery and identification of serum potential biomarkers for pulmonary tuberculosis using iTRAQ‐coupled two‐dimensional LC‐MS/MS

DD Xu, DF Deng, X Li, LL Wei, YY Li, XY Yang… - …, 2014 - Wiley Online Library
Pulmonary tuberculosis (TB) caused by Mycobacterium tuberculosis is a chronic disease.
Currently, there are no sufficiently validated biomarkers for early diagnosis of TB infection. In …

Combined proteomics and transcriptomics identifies carboxypeptidase B1 and nuclear factor κB (NF-κB) associated proteins as putative biomarkers of metastasis in …

P Bouchal, M Dvořáková, T Roumeliotis… - Molecular & Cellular …, 2015 - ASBMB
Current prognostic factors are insufficient for precise risk-discrimination in breast cancer
patients with low grade breast tumors, which, in disagreement with theoretical prognosis …

Multidimensional chromatography coupled to mass spectrometry in analysing complex proteomics samples

P Horvatovich, B Hoekman… - Journal of separation …, 2010 - Wiley Online Library
Multidimensional chromatography coupled to mass spectrometry (LCn‐MS) provides more
separation power and an extended measured dynamic concentration range to analyse …