[HTML][HTML] Structure and mechanism of the RNA polymerase II transcription machinery

AC Schier, DJ Taatjes - Genes & development, 2020 - genesdev.cshlp.org
Abstract RNA polymerase II (Pol II) transcribes all protein-coding genes and many
noncoding RNAs in eukaryotic genomes. Although Pol II is a complex, 12-subunit enzyme, it …

The role of FACT in managing chromatin: disruption, assembly, or repair?

T Formosa, F Winston - Nucleic acids research, 2020 - academic.oup.com
Abstract FACT (FAcilitates Chromatin Transcription) has long been considered to be a
transcription elongation factor whose ability to destabilize nucleosomes promotes RNAPII …

Structural basis of nucleosome disassembly and reassembly by RNAPII elongation complex with FACT

H Ehara, T Kujirai, M Shirouzu, H Kurumizaka… - Science, 2022 - science.org
During gene transcription, RNA polymerase II (RNAPII) traverses nucleosomes in chromatin,
but the mechanism has remained elusive. Using cryo–electron microscopy, we obtained …

[HTML][HTML] FACT maintains chromatin architecture and thereby stimulates RNA polymerase II pausing during transcription in vivo

K Žumer, M Ochmann, A Aljahani, A Zheenbekova… - Molecular Cell, 2024 - cell.com
Facilitates chromatin transcription (FACT) is a histone chaperone that supports transcription
through chromatin in vitro, but its functional roles in vivo remain unclear. Here, we analyze …

[HTML][HTML] Structure of a backtracked hexasomal intermediate of nucleosome transcription

L Farnung, M Ochmann, G Garg, SM Vos, P Cramer - Molecular Cell, 2022 - cell.com
During gene transcription, RNA polymerase II (RNA Pol II) passes nucleosomes with the
help of various elongation factors. Here, we show that RNA Pol II achieves efficient …

The N-terminus of Spt16 anchors FACT to MCM2–7 for parental histone recycling

X Wang, Y Tang, J Xu, H Leng, G Shi, Z Hu… - Nucleic acids …, 2023 - academic.oup.com
Parental histone recycling is vital for maintaining chromatin-based epigenetic information
during replication, yet its underlying mechanisms remain unclear. Here, we uncover an …

Spt6 directly interacts with Cdc73 and is required for Paf1 complex occupancy at active genes in Saccharomyces cerevisiae

MA Ellison, S Namjilsuren, MK Shirra… - Nucleic Acids …, 2023 - academic.oup.com
Abstract The Paf1 complex (Paf1C) is a conserved transcription elongation factor that
regulates transcription elongation efficiency, facilitates co-transcriptional histone …

Targeted protein degradation reveals a direct role of SPT6 in RNAPII elongation and termination

A Narain, P Bhandare, B Adhikari, S Backes, M Eilers… - Molecular cell, 2021 - cell.com
SPT6 is a histone chaperone that tightly binds RNA polymerase II (RNAPII) during
transcription elongation. However, its primary role in transcription is uncertain. We used …

Insights into Spt6: a histone chaperone that functions in transcription, DNA replication, and genome stability

CLW Miller, JL Warner, F Winston - Trends in Genetics, 2023 - cell.com
Transcription elongation requires elaborate coordination between the transcriptional
machinery and chromatin regulatory factors to successfully produce RNA while preserving …

SETD2: from chromatin modifier to multipronged regulator of the genome and beyond

TM Molenaar, F van Leeuwen - Cellular and Molecular Life Sciences, 2022 - Springer
Histone modifying enzymes play critical roles in many key cellular processes and are
appealing proteins for targeting by small molecules in disease. However, while the functions …