Evaluation of preferred binding regions on ubiquitin and IgG1 FC for interacting with multimodal cation exchange resins using DEPC labeling/mass spectrometry
There is significant interest in identifying the preferred binding domains of biological
products to various chromatographic materials. In this work, we develop a biophysical …
products to various chromatographic materials. In this work, we develop a biophysical …
Clear insight into complex multimodal resins and impurities to overcome recombinant protein purification challenges: A review
MM Goodarzi, R Jalalirad - Biotechnology and Bioengineering, 2025 - Wiley Online Library
Increasing attention has been paid to the purity of therapeutic proteins imposing extensive
costs and challenges to the downstream processing of biopharmaceuticals. One of the …
costs and challenges to the downstream processing of biopharmaceuticals. One of the …
[HTML][HTML] Predicting multimodal chromatography of therapeutic antibodies using multiscale modeling
R Hess, J Faessler, D Yun, A Mama, D Saleh… - … of Chromatography A, 2024 - Elsevier
Multimodal chromatography has emerged as a powerful method for the purification of
therapeutic antibodies. However, process development of this separation technique remains …
therapeutic antibodies. However, process development of this separation technique remains …
Molecular Dynamics Simulation of an Iron (III) Binding Site on the Fc Domain of IgG1 Relevant for Visible Light-Induced Protein Fragmentation
Molecular dynamics simulations were employed to investigate the interaction between Fe
(III) and an iron-binding site composed of THR259, ASP252, and GLU261 on the Fc domain …
(III) and an iron-binding site composed of THR259, ASP252, and GLU261 on the Fc domain …
Effect of Monovalent Cations on the Structure and Dynamics of Multimodal Chromatographic Surfaces
SC Lau, CL Bilodeau - Langmuir, 2024 - ACS Publications
While multimodal (MM) chromatography is a promising approach for purifying proteins, the
lack of a fundamental understanding of how ion–ligand interactions govern selectivity limits …
lack of a fundamental understanding of how ion–ligand interactions govern selectivity limits …
Exploring preferred binding domains of IgG1 mAbs to multimodal adsorbents using a combined biophysics and simulation approach
In this work, we employ a recently developed biophysical technique that uses
diethylpyrocarbonate (DEPC) covalent labeling and mass spectrometry for the identification …
diethylpyrocarbonate (DEPC) covalent labeling and mass spectrometry for the identification …
[HTML][HTML] Antibody sequence-based prediction of pH gradient elution in multimodal chromatography
R Hess, J Faessler, D Yun, D Saleh, JH Grosch… - … of Chromatography A, 2023 - Elsevier
Multimodal chromatography has emerged as a promising technique for antibody purification,
owing to its capacity to selectively capture and separate target molecules. However, the …
owing to its capacity to selectively capture and separate target molecules. However, the …
Biophysical Tools to Study Preferred Binding Domains for Protein Interactions with Chromatographic Surfaces
K Dhingra - 2023 - search.proquest.com
Multimodal resins have proven to be quite successful in removing various process and
product related impurities from protein therapeutics due to their ability to bind through …
product related impurities from protein therapeutics due to their ability to bind through …
[ΒΙΒΛΙΟ][B] Elucidating the Role of Multimodal Ligand Surfaces in Protein Chromatography Using Molecular Dynamics Simulations
M Vats - 2022 - search.proquest.com
Over the past several years, protein-based therapeutics have revolutionized the
pharmaceutical industry, with applications for treatment of a range of diseases from …
pharmaceutical industry, with applications for treatment of a range of diseases from …