The diversity of biomolecules present in plasmid DNA (pDNA)-containing extracts and the
structural and chemical similarities between pDNA and impurities are some of the main …

The increasing demand for highly pure biopharmaceuticals has put significant pressure on
the biotechnological industry to innovate in production and purification processes. Nucleic …

Background It is well known that the success of gene transfer to cells and subsequent
expression is strictly affected by the vector manufacturing process. Several challenges …

The aim of this study is to prepare supermacroporous pseudospecific cryogel which can be
used for the purification of plasmid DNA (pDNA) from bacterial lysate. N-methacryloyl-(l) …

The ability to isolate sc (supercoiled) pDNA (plasmid DNA) isoform should be one of the
features of a pDNA purification process, eliminating sample contaminants such as RNA …

The aim of this study is to prepare poly (hydroxyethyl methacrylate-N-methacryloyl-(L)-
histidine)[PHEMAH] magnetic nanoparticles for plasmid DNA (pDNA) purification from …

Arginine chromatography was used to fully separate supercoiled and open circular plasmid
DNA (pDNA) isoforms. The results show that the arginine matrix promotes multiple …

The development of efficient plasmid DNA (pDNA) purification processes has fostered
therapeutic applications like gene therapy and DNA vaccination. In fact, monolithic supports …

The development of a strategy to plasmid DNA (pDNA) purification has become necessary
for the development of gene therapy and DNA vaccine production processes in recent years …

Supercoiled state corresponds to the active form for plasmid applications. The relaxed
circular form of plasmids is often inactive or poorly active. To obtain significant amounts of …