Recent developments in bioprocessing of recombinant proteins: expression hosts and process development

NK Tripathi, A Shrivastava - Frontiers in Bioengineering and …, 2019 - frontiersin.org
Infectious diseases, along with cancers, are among the main causes of death among
humans worldwide. The production of therapeutic proteins for treating diseases at large …

Antibody‐incorporated nanomedicines for cancer therapy

SY Wu, FG Wu, X Chen - Advanced Materials, 2022 - Wiley Online Library
Antibody‐based cancer therapy, one of the most significant therapeutic strategies, has
achieved considerable success and progress over the past decades. Nevertheless …

Single-domain antibodies as versatile affinity reagents for analytical and diagnostic applications

G Gonzalez-Sapienza, MA Rossotti… - Frontiers in …, 2017 - frontiersin.org
With just three CDRs in their variable domains, the antigen-binding site of camelid heavy-
chain-only antibodies (HcAbs) has a more limited structural diversity than that of …

Solubility, stability, and avidity of recombinant antibody fragments expressed in microorganisms

TH Kang, BL Seong - Frontiers in microbiology, 2020 - frontiersin.org
Solubility of recombinant proteins (ie, the extent of soluble versus insoluble expression in
heterogeneous hosts) is the first checkpoint criterion for determining recombinant protein …

Microbial protein cell factories fight back?

LA Rettenbacher, K Arauzo-Aguilera, L Buscajoni… - Trends in …, 2022 - cell.com
The biopharmaceutical market is growing faster than ever, with two production systems
competing for market dominance: mammalian cells and microorganisms. In recent years …

Translatable high drug loading drug delivery systems based on biocompatible polymer nanocarriers

W Chen, S Zhou, L Ge, W Wu, X Jiang - Biomacromolecules, 2018 - ACS Publications
Most nanocarriers possess low drug loading, resulting in frequently repeated administration
and thereby high cost and increased side effects. Furthermore, the characteristics of …

Single‐chain fragment variable produced by phage display technology: Construction, selection, mutation, expression, and recent applications in food safety

L Li, S Wu, Y Si, H Li, X Yin… - Comprehensive Reviews in …, 2022 - Wiley Online Library
Immunoassays are reliable, efficient, and accurate methods for the analysis of small‐
molecule harmful substances (such as pesticides, veterinary drugs, and biological toxins) …

Antibody fragments as potential biopharmaceuticals for cancer therapy: success and limitations

RV Kholodenko, DV Kalinovsky… - Current Medicinal …, 2019 - ingentaconnect.com
Monoclonal antibodies (mAbs) are an important class of therapeutic agents approved for the
therapy of many types of malignancies. However, in certain cases applications of …

Glycosylation control technologies for recombinant therapeutic proteins

SK Gupta, P Shukla - Applied microbiology and biotechnology, 2018 - Springer
Protein glycosylation is a very important quality attribute of any biopharmaceutical product
as it affects the efficacy, serum half-life, and antigenicity of a molecule. The present …

An Alkaline Protease from Bacillus pumilus MP 27: Functional Analysis of Its Binding Model toward Its Applications As Detergent Additive

M Baweja, R Tiwari, PK Singh, L Nain… - Frontiers in …, 2016 - frontiersin.org
A proteolytic strain of Bacillus pumilus MP 27 was isolated from water samples of Southern
ocean produced alkaline protease. Since protease production need expensive ingredients …