Understanding metabolism with flux analysis: From theory to application
Quantitative and qualitative knowledge of metabolic rates (ie fluxes) over a metabolic
network and in specific cellular compartments gives insights into the regulation of …
network and in specific cellular compartments gives insights into the regulation of …
Hairy root cultures: a suitable biological system for studying secondary metabolic pathways in plants
P Sharma, H Padh… - Engineering in Life …, 2013 - Wiley Online Library
Hairy roots, a plant disease caused by Agrobacterium rhizogenes, show distinctive features
such as high growth rate, unlimited branching, and biochemical and genetic stability. Hairy …
such as high growth rate, unlimited branching, and biochemical and genetic stability. Hairy …
Ensemble modeling of metabolic networks
LM Tran, ML Rizk, JC Liao - Biophysical journal, 2008 - cell.com
Complete modeling of metabolic networks is desirable, but it is difficult to accomplish
because of the lack of kinetics. As a step toward this goal, we have developed an approach …
because of the lack of kinetics. As a step toward this goal, we have developed an approach …
Measuring multiple fluxes through plant metabolic networks
Fluxes through metabolic networks are crucial for cell function, and a knowledge of these
fluxes is essential for understanding and manipulating metabolic phenotypes. Labeling …
fluxes is essential for understanding and manipulating metabolic phenotypes. Labeling …
Towards model-driven characterization and manipulation of plant lipid metabolism
Plant lipids have versatile applications and provide essential fatty acids in human diet.
Therefore, there has been a growing interest to better characterize the genetic basis …
Therefore, there has been a growing interest to better characterize the genetic basis …
Tracking the metabolic pulse of plant lipid production with isotopic labeling and flux analyses: past, present and future
Metabolism is comprised of networks of chemical transformations, organized into integrated
biochemical pathways that are the basis of cellular operation, and function to sustain life …
biochemical pathways that are the basis of cellular operation, and function to sustain life …
Opportunities in metabolic engineering to facilitate scalable alkaloid production
E Leonard, W Runguphan, S O'connor… - Nature chemical …, 2009 - nature.com
Numerous drugs and drug precursors in the current pharmacopoeia originate from plant
sources. The limited yield of some bioactive compounds in plant tissues, however, presents …
sources. The limited yield of some bioactive compounds in plant tissues, however, presents …
Quantification of compartmented metabolic fluxes in develo** soybean embryos by employing biosynthetically directed fractional 13C labeling, two-dimensional …
Metabolic flux quantification in plants is instrumental in the detailed understanding of
metabolism but is difficult to perform on a systemic level. Toward this aim, we report the …
metabolism but is difficult to perform on a systemic level. Toward this aim, we report the …
Metabolic flux analysis of CHO cells in perfusion culture by metabolite balancing and 2D [13C, 1H] COSY NMR spectroscopy
C Goudar, R Biener, C Boisart, R Heidemann… - Metabolic …, 2010 - Elsevier
The physiological state of CHO cells in perfusion culture was quantified by determining
fluxes through the bioreaction network using 13C glucose and 2D-NMR spectroscopy. CHO …
fluxes through the bioreaction network using 13C glucose and 2D-NMR spectroscopy. CHO …
Metabolic network fluxes in heterotrophic Arabidopsis cells: stability of the flux distribution under different oxygenation conditions
Steady-state labeling experiments with [1-13C] Glc were used to measure multiple metabolic
fluxes through the pathways of central metabolism in a heterotrophic cell suspension culture …
fluxes through the pathways of central metabolism in a heterotrophic cell suspension culture …