SpyMask enables combinatorial assembly of bispecific binders

CL Driscoll, AH Keeble, MR Howarth - Nature Communications, 2024 - nature.com
Bispecific antibodies are a successful and expanding therapeutic class. Standard
approaches to generate bispecifics are complicated by the need for disulfide …

Visualizing heterogeneous protein conformations with multi-tilt nanoparticle-aided cryo-electron microscopy sampling

C Kim, Y Kim, SJ Lee, SR Yun, J Choi, SO Kim… - Nano Letters, 2023 - ACS Publications
Obtaining the heterogeneous conformation of small proteins is important for understanding
their biological role, but it is still challenging. Here, we developed a multi-tilt nanoparticle …

[HTML][HTML] Characterizing protein–surface and protein–nanoparticle conjugates: Activity, binding, and structure

JM Correira, PR Handali, LJ Webb - The Journal of Chemical Physics, 2022 - pubs.aip.org
Many sensors and catalysts composed of proteins immobilized on inorganic materials have
been reported over the past few decades. Despite some examples of functional protein …

[HTML][HTML] SpyCombinator Assembly of Bispecific Binders

CL Driscoll, AH Keeble, M Howarth - bioRxiv, 2023 - biorxiv.org
Bispecific antibodies are a successful and expanding therapeutic class, bridging two cell-
types or engaging two different molecules on the same cell. Bispecifics unlock avenues …

Visualizing Heterogeneous Protein Conformations with Multi-Tilt Nanoparticle-Aided Cryo-Electron Microscopy Sampling

Y Kim, C Kim, SJ Lee, SR Yun, J Choi, SO Kim, Y Yang… - 2024 - academic.oup.com
Studying heterogeneous conformations of small proteins in solution is essential for
understanding their role in biological processes, but achieving this using traditional methods …

Observing Conformational Distribution of Protein using Nanoparticle-Aided Cryo-Electron Microscopy Sampling

Y Kim, C Kim, Y Yang, H Ihee - 13th Asia Pacific Microscopy …, 2025 - scienceopen.com
Characterizing the structural heterogeneity of small proteins is essential for understanding
their biological roles. Conventional methods have been used to investigate the …