Microglial research has advanced considerably in recent decades yet has been constrained
by a rolling series of dichotomies such as" resting versus activated" and" M1 versus M2." …

Optogenetic techniques have been developed to allow control over the activity of selected
cells within a highly heterogeneous tissue, using a combination of genetic engineering and …

Astrocytes, a type of glia, are abundant and morphologically complex cells. Here, we report
astrocyte molecular profiles, diversity, and morphology across the mouse central nervous …

Cell types are the basic functional units of an organism. Cell types exhibit diverse
phenotypic properties at multiple levels, making them challenging to define, categorize, and …

Single-cell RNA sequencing (scRNA-seq) is a powerful tool for defining cellular diversity in
tumors, but its application toward dissecting mechanisms underlying immune-modulating …

The gene-regulatory landscape of the brain is highly dynamic in health and disease,
coordinating a menagerie of biological processes across distinct cell types. Here, we …

A key aspect of nearly all single-cell sequencing experiments is dissociation of intact tissues
into single-cell suspensions. While many protocols have been optimized for optimal cell …

In the past decade, single-cell technologies have proliferated and improved from their
technically challenging beginnings to become common laboratory methods capable of …

Cellular perturbations underlying Alzheimer's disease (AD) are primarily studied in human
postmortem samples and model organisms. Here, we generated a single-nucleus atlas from …

Single-cell RNA sequencing (scRNA-seq) allows researchers to collect large catalogues
detailing the transcriptomes of individual cells. Unsupervised clustering is of central …